深圳市盐田区人群肠道病毒及常见感染性腹泻病毒调查分析

目的 了解深圳市盐田区人群肠道病毒及常见感染性腹泻病毒隐性感染状况,为制定防控措施提供科学依据。 方法 2020 年在盐田区海山、沙头角、盐田、梅沙四个街道,分别整群随机抽取 1 个社区为调查点,采集居民粪便样本用实时荧光定量 PCR 方法检测诺如病毒、札如病毒、轮状病毒、星状病毒、肠道腺病毒、肠道病毒通用型、肠道病毒 71 型(EV71)和柯萨奇病毒 A16 型(CV-A16)。 结果 共收集并检测粪便样品 813 份,未检出札如病毒、轮状病毒、星状病毒、肠道腺病毒、EV71 和 CA16;检出肠道病毒通用型 2 例,阳性率为 0. 25%,均为幼童口服脊髓灰质炎疫苗所致;检出诺如病毒 4 例,均为诺如 GⅡ型,阳性率为 0. 49%。 4 例诺如病毒阳性样本成功测序 2 例,分别为 GⅡ. 2-GⅡ. P16 型和 GⅡ. 17-GⅡ. P17 型,均与近年国内流行株有较高同源性。 结论 深圳市盐田区人群存在诺如病毒隐性感染,应加强对重点人群、重点场所的监测。

Investigation on the enterovirus and infectious diarrhea virus in population in Yantian district of Shenzhen

Objective To understand the status of recessive infection of enterovirus and infectious diarrhea virus in population in Yantian district of Shenzhen City for scientific evidence to formulate prevention and control measures. Methods In 2020,one community was randomly selected by clustering method as survey site from each of the four blocks (Haishan,Shatoujiao,Yantian and Meisha) under administration of Yantian District. Fecal samples were collected from local residents, and detected with real-time fluorescence quantitative PCR for norovirus,zavirus,rotavirus,star virus,intestinal adenovirus, intestinal universal type,enterovirus 71 (EV 71) and coxsackievirus A16 (CV-A16). Results Totally,813 aliquots of fecal samples were collected and tested,in which no zavirus,rotavirus,star virus,intestinal adenovirus,EV 71 and CA16 were detected. Universal type of human entrovirus,which was associated with oral polio vaccine,was found in 2 children,with a positive rate of 0. 25%. Norovirus(genogroup Ⅱ) was detected in 4 cases,with a positive rate of 0. 49%. Two out of the 4 norovirus positive samples were successfully sequenced,which were confirmed as genotype GⅡ. 2-GⅡ. P16 and GⅡ. 17-G Ⅱ. P17,and had higher homology with the strains prevalent in China in recent years. Conclusion Recessive infection of norovirus exists in the population in Yantian district of Shenzhen City. The findings suggest that surveillance should be strengthened key population and areas.