普罗布考对糖尿病大鼠大血管病变的影响

目的 探讨普罗布考对糖尿病大鼠大血管病变的影响及机制.方法 44只健康Sprague Dawley(SD)雄性大鼠随机分为对照组(NC组,n=8)、糖尿病组(DM组,n=18)、普罗布考干预组(PB组,n=18)3组,四氧嘧啶50 mg/kg行尾静脉注射制备糖尿病大鼠模型.糖尿病造模成功后PB组给予普罗布考170 mg...

Effects of probucol on macro-vasculopathy of diabetic rats

Objective To investigate the intervention and mechanism of probucol for macro-vasculopathy of diabetic rats. Methods Forty-four healthy Sprauge-Dawley (SD) male rats were randomly divided into three groups:normal control group (NC group,n =8) ,diabetes mellitus group (DM group,n=18) ,probucol intervention group ( PB group, n = 18 ). The diabetic rat models made by the vena caudalis were injected with alloxan (50 mg/kg weight). The rats of PB group were given intragastric administration with probucol 170 mg/( kg · d) for three weeks. At the end of the fourth week, the rats were killed. Before that serum glucose, lipid, weiglt and malodialdehyde ( M DA ), superoxide dismutase ( SOD), catalase (CAT), glutathione peroxidase(GSH-PX) were tested. The architectonic appearance of arota were investigated. The expression of the monocyte chemoattactant protein-1 ( MCP-1 ) and intercellular adhension molecule-1 ( ICAM-1 ) were detected by immunohistoohemical staining. Results ( 1 ) Compared with NC group, the weight, serum glucose and low density lipoprotein-cholesterol (LDL-C) in DM group were much higher( P ＜0.05 ). Compared with DM group,the weight in PB group were higher (P＜0. 05),but the serum glucose and LDL-C were lower (P ＜0.05). (2)Compared with NC group,the MDA and GSH-PX in DM group and PB group were much higher( P ＜ 0.05 ), but the SOD/MDA and CAT were much lower( P ＜ 0.05 ). Compared with DM group, the MDA of PB group were much higher( P ＜ 0. 05 ), but the SOD/MDA and CAT were much lower( P ＜ 0. 05 ).(3) The endothelial cells of the arota of diabetic rats were desquamated heavily, so that the endomembrane of the arota were not integrated. Therefore, the inflammatory cells were infiltrated under the endomembrance, the smooth muscle cells of the stratum medium were hyperplasia and chaos. There were no change in NC group,while little change in PB group. (4)The expression of MCP-1 and ICAM-1 in NC group were weak,but in DM group were strong. Furthermore,the expression of MCP-1 and ICAM-1 in PB group were weaker than that in DM group. Conclusion Probucol can improve the macro-vasculopathy of diabetic rats by reducing the levels of oxidative stress and LDL-C.