硫代乙酰胺诱导的A型肝性脑病小鼠焦虑样反应观察

目的探讨硫代乙酰胺(TAA)诱导的A型肝性脑病小鼠的精神障碍。方法 30只雄性昆明种小鼠随机分为实验组15只,对照组15只。实验组采用TAA 200 mg·kg-1·d-1,连续腹腔注射3 d至累积计量到600 mg/kg,正常对照组腹腔注射生理盐水,造模24 h后进行脑功能评分、明暗箱、旷场及高架十字实验。行为学检测结束后24 h行血氨及肝脏生化指标检测。两组间比较采用独立样本t检验。结果实验组小鼠在明暗箱实验中的明箱滞留时间(t=-4.006,P0.01)与穿梭次数(t=-2.656,P0.05);旷场实验的中央活动路程(t=-3.639,P0.05)、中央活动时间(t=-2.294,P0.05)、垂直运动次数(t=-2.282,P0.05)、理毛时间(t=5.992,P0.01);高架十字实验的开臂进入次数(t=-3.584,P0.05)、开臂滞留时间(t=-3.992,P0.05)与对照组小鼠相比,差异均具有统计学意义。但旷场实验中两组小鼠活动总路程差异无统计学意义(t=-0.96,P0.05)。且实验组小鼠血氨(t=-3.168,P0.05)、ALT(t=4.316,P0.05)、AST(t=-2.581,P0.05)、TBil(t=-9.127,P0.01)水平明显大于对照组,差异均有统计学意义。结论用TAA腹腔注射建立的A型肝性脑病小鼠模型有焦虑样反应;小剂量TAA诱导A型肝性脑病小鼠的焦虑样反应不伴有自发活动能力下降。

Anxiety in mice with acute hepatic encephalopathy induced by thioacetamide

Objective To evaluate the mental disorders in mice with thioacetamide （TAA）-induced acute hepatic encephalopathy （AHE）. Methods Thirty male Kunming mice were equally and randomly divided into two groups （treatment group and control group）.Mice in the treatment group received an intraperitoneal injection of TAA （200 mg·kg -1·d -1 for 3 days,up to 600 mg·kg -1 ）,as compared with the same volume of physiological saline in the control group.Neurological function score,light/dark box,open field,and elevated plus -maze test were used to determine behavioral parameters at 24 h after the model was established.Serum ammonia,aspartate amino transferase （AST）,al-anine aminotransferase （ALT）,and total bilirubin （TBil）were measured at 24 h post determination of behavioral parameters.Comparison be-tween two groups was made by independent -samples t test.Results In the light/dark box test,the treatment group had significantly reduced residence time in the light box and number of shuttles between two boxes （t =-4.006,P 〈0.01;t =-2.656,P 〈0.05）;in the open field test,the treatment group had significantly reduced central distance,central time,and number of vertical movements （t =-3.639,P 〈0.05;t =-2.294,P 〈0.05;t =-2.282,P 〈0.05）,as well as increased grooming time （t =5.992,P 〈0.01）;meanwhile,the number of open-arm entries and residence time in the open arm for the treatment group were also reduced in the elevated plus -maze test,as compared with the control group （t =-3.584,P 〈0.05;t =-3.992,P 〈0.05）.However,the total distance in the open field test showed no significant difference between the two groups （t =-0.96,P 》0.05）.Moreover,compared with the control group,the treatment group had significant-ly higher levels of serum ammonia （t =-3.168,P 〈0.05）,ALT （t =4.316,P 〈0.05）,AST （t =-2.581,P 〈0.05）,and TBil （t =-9.127,P 〈0.01）.Conclusion Mice with AHE induced by an intraperitoneal injection of low -dose TAA have anxiety -related behav-iors