血小板自身抗体的流式细胞仪检测与意义

目的：应用流式细胞术（ＦＣＭ）测定血小板自身抗体ＰＡＩｇＧ，研究其对待发性血小板减少性紫癜（ＩＴＰ）诊断的意义。方法：应用ＦＣＭ检测２０例ＩＴＰ患者血小板膜表面及血浆中的抗血小板抗体，并与ＥＬＩＳＡ所测结果进行比较。结果：ＦＣＭ测得１４例（７０％ＩＴＰ患者血小板膜自身抗体ＰＡＩｇＧ阳性，９例（４５％）血浆抗血小板抗体阳性。ＥＬＩＳＡ显示１２例（６０％）患者ＰＡＩｇＧ阳性，７例（３５％）血浆抗血小板

Flowcytometry study of platelet associated antibody in patient s with idiopathic thrombocytopenic pupura

Objective:Set up a method of measuring platelet associated antibody PAIgG by flowcytometry and investigate its significance in the diagnosis of idiopathic thrombocytopenic purpura (ITP).Method:With flowcytometry, PAIgG on the platelet membrane and in plasma were measured in 20 patients with ITP, which were compared with the result of ELISA.Result:By flowcytometry, the PAIgG on platelet membrane was positive in 14 ITP cases(70%), but in plasma, the PAIgG was positive in 9 cases(45%).The samples of theses patients were also measured with ELISA, which idicated positive platelet PAIgG in 12 cases (60%)and positive plasma PAIgG positive in 7 cases(35%). Compared with the results of ELISA, the positive percentage of PAIgG measured by FCM in ITP patients seems higher.Conclusion:FCM can be used as a new, rapid and simple method in detecting platelet autoantibody. FCM, in combination with ELISA, would improve the reliability and the positive percentage of PAIgG measurement in ITP patients.