| 鼠疫菌6MD质粒对假结核菌外膜蛋白的影响 |
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| 引用本文： | 王芳,刘志奇.鼠疫菌6MD质粒对假结核菌外膜蛋白的影响[J].中国地方病学杂志,1992,11(6):342-345,394. |
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| 作者姓名： | 王芳 刘志奇 |
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| 作者单位： | 中国预防医学科学院流行病学微生物学研究所
(王芳，刘志奇，景怀琦)，中国预防医学科学院流行病学微生物学研究所(俞东征) |
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| 摘 要： | 检查了来自鼠疫耶尔森氏菌 Y·pestis 的6MD 质粒转入假结核耶尔森氏菌 Y·(?)berculosis 后,对后者表达的耶氏菌属外膜蛋白(Yops)的影响。研究表明,6MD 质粒所编码的 pla 基因产物可以降解这些外膜蛋白,同时,也能降解假结核菌的 YadA 蛋白,使它转变为几种分子量略小的蛋 质亚单位或片断。本文还讨论了这种现象对鼠疫菌和假结核菌毒力的影响。
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| 关 键 词： | 鼠疫菌 假结核菌 外膜蛋白 |
The effect of 6-MD plasmid of Yersinia pestis on the OMPs of Yersinia pseudotuberculosis |
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| Wang Fang.The effect of 6-MD plasmid of Yersinia pestis on the OMPs of Yersinia pseudotuberculosis[J].Chinese Jouranl of Endemiology,1992,11(6):342-345,394. |
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| Authors: | Wang Fang |
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| Abstract: | The 6-MD plasmid of Yersinia pestis was cloned into PBR328,and then was introduced into Yersinia pseudotu-berculosis by electroporation.With the transforments have been obtained,the influence of the 6-MD plasmid on the expression of YOPs of Yersinia pseudotuberculosis was investigated.The results showed that the pla gene products en-coded by the 6-MD plasmid could digest these outer membranes(YOPs)as well as YadA that expressed in parents ofYersinia pseudotuberculosis.On SDS-PAGE YadA was changed into several sub-units or fragments with smallermolerular weight.The effect of this phenomenon in the virulence of Yersinia pestis and Yersinia pseudotuberculosiswas discussed in this study. |
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| Keywords: | outer-membrane protein Yops yadA SDS-PAGE |
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