低硒低维生素E诱导肝细胞凋亡及cAMP和细胞内Ca2+的作用

目的 研究低硒（Ｓｅ）低维生素Ｅ（ＶＥ）能否诱导大鼠肝细胞凋亡及ｃＡＭＰ和细胞内Ｃａ＾２＋所起的作用。方法 以天然的和人工半合成的低Ｓｅ低ＶＥ饲料喂养大鼠１７周，采用流式细胞术检测肝细胞凋亡，采用放免法检测ｃＡＭＰ含量，Ｆｕｒａ－２负载荧光分光光度法测定细胞内Ｃａ＾２＋含量。结果 与补Ｓｅ和ＶＥ组大鼠相比，低Ｓｅ低ＶＥ组大鼠肝细胞凋亡显著增加；ｃＡＭＰ含量明显升高；细胞内Ｃａ＾２＋含量明显升高。结

Hepatocyte apoptosis induced by selenium and vitamin E deficiency and the role of cAMP and Ca2+ in the apoptotic process

Objective To study whether dietary deficiency of selenium (Se) and vitamine E (VE) can induce hepatocyte apoptosis and what is the role of cAMP and Ca 2 in the apoptotic process.Methods The experiment was performed on rats kept on natural or semisynthetic diet deficient in Se and VE for 17 weeks.The hepatocyte apoptosis was detected by using flow cytometry in rats.cAMP was detected by using radioimmunoassay,intracellular free Ca 2 was assayed by fluorescence spectrophotometry after the hepatocytes were loaded with a fluorescence probe (Fura 2AM).Results As compared with Se and VE supplemented groups,hepatocyte apoptosis was obviously increased in rats kept on Se and VE deficient diet,accompanied with an increase of cAMP and Ca 2 .Conclusions The dietary deficiency of Se and VE can induce hepatocyte apoptosis in rats;cAMP and Ca 2 may be involved in the regulation of the apoptotic process.