江西省高安市两株汉城病毒分离鉴定及全基因组序列分析

目的 对2021年江西省高安市采集的鼠肺样本进行肾综合征出血热汉坦病毒分离鉴定及全基因组序列进行分析.方法 采用Vero-E6细胞分离肾综合征出血热汉坦病毒,采用实时荧光RT-PCR法和直接免疫荧光法对分离株进行鉴定,采用高通量测序技术对分离株进行全基因组测序,采用CLC、MEGA7.0等软件对基因组序列进行分析.结果...

Isolation and complete genome sequence analysis of two Seoul orthohantavirus strains isolated from Gaoan City,Jiangxi Province

This study aimed to analyze whole genome sequences of Seoul orthohantavirus from rat lung samples collected in Gaoan City, Jiangxi Province in 2021. Vero-E6 cells were used to isolate orthohantavirus; real-time fluorescent RT-PCR and direct immunofluorescence were used to identify the isolates, high-throughput sequencing technology was used to sequence the whole genomes of the isolates, and CLC and MEGA7.0 software were used to analyze the genome sequence. Two strains of SEOV, named GAW30/2021 and GAW50/2021, were isolated from Rattus norvegicus and Rattus rosenbergii, respectively. The three gene fragments of the two strains were the same size, and the length of the coding region of each gene was the same. The S, M and L genes of the isolates contained 1 773, 3 651 and 6 530 nucleotides, encoding 429, 1 133 and 2 151 amino acids, respectively. The S, M and L nucleotide sequence identity of the two isolates was 99.7%, 99.0% and 99.4%, and the amino acid sequence identity was 99.8%, 99.7% and 99.9%, respectively. The two strains shared 84.3%-94.9% nucleotide sequence identity and 96.1%-99.8% amino acid sequence identity with SEOV reference strains. The amino acid sequence identity between the two strains and the SEOV vaccine strain was 97.6%-99.1%. The amino acid sequence of nucleoprotein and glycoprotein epitopes of the two strains was consistent with that of the vaccine strain. This study reports the first isolation of SEOV strains from rat lung samples in Gaoan City, Jiangxi Province, and indicates that the current vaccine still has protective effects against variants.