2014-2020年宁夏沙门菌分子分型与耐药性研究北大核心CSCD

目的分析2014-2020年宁夏食品及病例来源沙门菌耐药情况、流行优势血清型及PFGE型别。方法对2014-2020年各类食品及临床病例中分离培养的188株沙门菌进行血清分型、脉冲场凝胶电泳(PFGE)分子分型和肉汤稀释法药物敏感性实验。结果肠炎沙门菌在食品及病例中均为优势血清型,病例来源分离76株,占49.35%(76/154),食品来源分离18株,占52.94%(18/34),总构成比为50%(94/188)。188株不同来源沙门菌有182株出现不同程度的耐药,176株为多重耐药菌株。其中耐2~4种抗生素101株(55.49%),耐5~8种抗生素52株(28.57%),耐9~12种抗生素23株(6.59%)。将所有分离株经Xbal酶切,脉冲场凝胶电泳后,共获得93条PFGE带型,相似度为42.1%~97.1%,优势带型为NXSM0065型,其次为NXSM0074和NXSM0069,共41株,占所有分离株的21.81%。结论宁夏地区沙门菌流行优势血清型为肠炎沙门菌,病例及食品来源分离株耐药状况普遍较为严重,不同来源,同一血清型PFGE带型同源性较高,且与耐药谱未体现出明显的关联性。

Molecular typing and drug resistance of Salmonella in Ningxia from 2014 to 2020

This study aimed to analyze the drug resistance, prevalent serotypes and pulsed field gel electrophoresis (PFGE) types of Salmonella from food and case sources in Ningxia from 2014 to 2020. Serum typing, PFGE typing and broth dilution drug sensitivity tests were performed on 188 strains of Salmonella isolated from various foods and clinical cases from 2014 to 2020. Salmonella enteritidis was the predominant serotype in foods and clinical cases. A total of 76 strains (49.35%) and 18 strains (52.94%) were isolated from food, accounting for 50% (94/188). Among 188 Salmonella strains from various sources, 182 strains showed different levels of drug resistance, and 176 strains were multi-drug resistant. Among them, 101 strains (55.49%) were resistant to two to four antibiotics, 52 strains (28.57%) were resistant to five to eight antibiotics, and 23 strains (6.59%) were resistant to 9-12 antibiotics. After Xbal restriction enzyme digestion, 93 PFGE bands were obtained, and the similarity was 46.3%-97.0%. The dominant bands were NXSM0065, NXSM0074 and NXSM0069, representing 41 strains accounting for 21.81% of all isolates. The predominant serotype of Salmonella in Ningxia was Salmonella enteritis, and the drug resistance of clinical cases and food-derived isolates was generally severe. The same serotype of PFGE from different sources showed high homology and did not show an obvious correlation with the drug resistance spectrum.