海南省西部地区莱姆病血清学调查

目的 了解海南省西部地区关节炎或神经性疾病患者的莱姆病感染状况。方法 在东方市收集莱姆病疑似病例血清400份,儋州市收集莱姆病疑似病例血清500份,均为关节炎或神经性疾病患者。采用间接免疫荧光法(IFA)对血清标本进行莱姆病抗体初筛检测;并采用免疫印迹法(WB)对IFA检测阳性的血清标本进行确诊实验。结果 IFA方法检测血清样本900份,35份阳性,阳性率为3.89％。应用 WB法对上述35份IFA阳性血清样本进行检测,结果15份阳性, 阳性率为1.67％。不同年龄人群均有莱姆病螺旋体感染,其中,≤20岁阳性率最高,为11.76%(4/34),差异有统计学意义(χ²=24.108,P<0.001);莱姆病螺旋体感染在性别和症状间差异无统计学意义 (χ²=0.001,P=0.975;χ²=0.011,P=0.917)。结论 证实了海南省西部地区人群中存在莱姆病,建议当地医生在诊治关节炎和神经性疾病患者时,应排除莱姆病的可能。

Serological investigation on Lyme disease in Western Region of Hainan Province

To investigate the infection status of Lyme spirochete in patients with arthritis or neurological disease in Western Region, Hainan Province. 400 suspected cases of Lyme disease were collected in Dongfang City, and 500 suspected cases of Lyme disease were collected in Zhangzhou City, all of which were patients with arthritis or neurological diseases. Serum samples were tested for initial screening of Lyme disease by Indirect Fluorescent-Antibody Test(IFA); serum samples positive for IFA were confirmed by Western blot (WB). Results showed that 35 in a total of 900 serum samples were tested positive by IFA, with the positive rate of 3.89%. In the 35 IFA-positives, 15 were confirmed as positive by WB, with positive rate of 1.67%. There were Lyme disease spirochete infections in different age groups were observed. Among them, The highest positive rate was 11.76% (4/34)in the group under 20 years old, with statistically significance (χ²=24.108, P<0.001),while no significant statistical difference of infection rate between gender(χ²=0.001,P=0.975) and symptoms (χ²=0.011,P=0.917) were found. This research confirmed the existence of human Lyme disease in Western Region, Hainan Province. We suggested that Lyme disease should be commonly considered by clincians when the patients had some related symptoms of Lyme disease in the Western Region, Hainan Province.