Smad2及P38MAPK在泡球蚴病灶旁肝脏组织中的表达及意义

目的 观察在泡球蚴肝脏组织中Smad2和P38MAPK的表达,并分析二者在肝纤维化进程中的作用及其相互关系.方法 将20例经手术治疗的肝泡状棘球蚴病肝组织匹配后分为邻近病变的组织(即病灶旁)与正常肝组织,利用免疫组织化学的方式检测Smad2及P38MAPK的表达情况.HE和Masson染色,光镜下观察病理改变与肝纤维化程度.结果 泡球蚴肝脏病灶旁组织比正常肝组织纤维化程度高;Smad2和P38MAPK在病灶旁组织均比正常肝组织的表达明显较高;Smad2和P38MAPK无论在正常肝组织,病灶肝组织,还是整个肝组织中,其表达水平均与纤维化程度正相关;随着纤维化程度的加重,Smad2和P38MAPK的表达水平也都相应增加;病灶旁组织中Smad2和P38MAPK的表达成正相关.结论 泡球蚴肝脏病灶旁组织比正常肝组织纤维化程度明显严重;Smad2和P38MAPK均参与促进泡球蚴致肝纤维化的机制;在病灶旁组织中,P38MAPK参与调控对Smad2的正向调节.

Expression and significance of Smad2 and P38MAPK in hepatic tissues next to Echinococcus multilocularis

To observe the expression level of Smad2 and P38MAPK protein in liver tissues of human with Echinococcus multilocularis (E. m), and to evaluate their interaction and the mechanism of signals in hepatic fibrosis, 20 patients who were diagnosed with Alveolar echinococcosis (AE) through surgery was recruited and their hepatic tissues were divided into para-lesion and normal liver tissues; IHC was applied to detect the expression of Smad2 and P38MAPK. Pathologic changes and the degree of hepatic fibrosis were evaluated using HE and Masson staining. Result showed that the scores of HF in para-lesions was higher than that in normal liver tissues; the expression level of Smad2 and P38MAPK in para-lesion were both higher than that in normal liver tissues; the expression level of Smad2 and P38MAPK in normal liver tissues, para-lesion or the whole hepatic tissues were all positively correlated with the degree of fibrosis; the expression level of Smad2 and P38MAPK increased with the degree of fibrosis; the expression level of Smad2 was positively correlated with that of P38MAPK in para-lesion. In conclusion, the degree of fibrosis in para-lesion with E. m is more serious than that in normal liver tissues; Smad2 and P38MAPK are involved in the positive process of liver fibrosis; P38MAPK is involved in the positive regulation of Smad2.