鹧鸪禽流感病毒和人流感病毒唾液酸受体分布特征

目的 探讨鹧鸪禽流感病毒和人流感病毒唾液酸受体分布特点及其作用。方法 用亲和组化法检测鹧鸪呼吸道与消化道流感病毒唾液酸受体的分布,荧光素Alexa488标记禽流感病毒H9N1和人流感病毒H1N1,分别与鹧鸪呼吸道、消化道各解剖部位组织细胞结合。结果 鹧鸪呼吸道同时表达SAα-2,3Gal和SAα-2,6Gal两种受体,但其消化道仅表达SAα-2,3Gal受体。SAα-2,3Gal受体在鹧鸪呼吸道各部位和消化道结肠上皮细胞呈强阳性弥漫分布;SAα-2,6Gal受体在呼吸道气管、支气管和次级支气管呈强阳性弥漫分布,而副支气管、消化道结肠均为缺乏或仅表达极少量。H9N1禽流感病毒与鹧鸪呼吸道各部位和消化道结肠均结合;人流感病毒H1N1与鹧鸪的气管、支气管、次级支气管结合,但未见与副支气管和结肠结合,此结果与鹧鸪呼吸道和消化道SAα-2,3Gal和SAα-2,6Gal受体分布总体一致。结论 鹧鸪同时表达SAα-2,3Gal和SAα-2,6Gal受体,能与禽流感病毒和人流感病毒结合,有助于禽流感病毒与人流感病毒基因重配,提示其可充当流感病毒潜在的中间宿主。

Distribution characteristics of avian influenza virus and human influenza virus sialic acid receptors in chukar

The distribution characteristics of influenza viruses receptors in chukar was studied, and whether it possess the proper characteristics for serving as an intermediate host for the transmission of influenza viruses from avian species to human was investigated in our research. Lectin histochemieal staining and virus binding test were used and results showed that both the SAα-2,3Gal receptors and SAα-2,6Gal receptors were expressed in respiratory tract, but only the SA α-2,3Gal receptors was expressed in chukar alimentary tract. The SAα2,3Gal receptors was general presence in the trachea, bronchus, second bronchus, parabrochus, and colon of chukar. The SAα2,6Gal receptors was prevalent in the trachea, bronchus and second bronchus, but was infrequent in parabrochus and colon of chukar. Furthermore, Alexa 488 labelled avian influenza H9N1 virus could hind to epithelial cells in respiratory tract and alimentary tract of chukar. Human influenza H1N1 virus could only bind to the trachea, bronchus and second bronchus, but not to the parabrochus and colon of chukar. Taken together, these results indicate that chukar, which carry both SAα2,3Gal and SAα2,6Gal receptors compatible with binding of avian and human influenza viruses, could provide an environment of reassortants between avian and human influenza viruses and act as potential intermediate hosts for avian influenza virus transmission to humans and could generate new influenza viruses with pandemic potential.