日本血吸虫SIEA26-28kDa的抗雌虫生殖免疫作用--HGPRT是否是主要的靶抗原

目的观察日本血吸虫SIEA26—28kDa抗原的抗雌虫生殖作用。方法用AcA柱层析纯化日本血吸虫早期胚卵中的SIEA和SIEA26—28kDa成分。并以此纯化产物免疫BALB／c小鼠。将小鼠分为三组，分别用SIEA、SIEA26—28kDa和免疫佐剂免疫动物，然后进行尾蚴攻击感染。46天后剖杀动物，冲虫，然后作虫荷测定及虫卵分类计数。结果SIEA26—28kDa免疫组雌虫子宫内减卵率为54．8％。与对照组比较有极显著性差异；该实验组动物肝脏和小肠组织内的虫卵数比对照组分别降低了48.07％和77.41％，且以成熟虫卵数的下降较为显著，分别降低了83．6％和93．3％；粪卵数的下降幅度最为显著，达87．26％。结论SIEA26—28kDa可作为一种抗卵胚发育及抗雌虫生殖的候选抗原分子。

Anti-fecundity immunity induced by sIEA 26-28kDa antigens of Schistosoma japonicum:is HGPRT antigen a major target for the immunity?

To study the anti fecundity effect of SIEA 26 28 kDa antigens from Schistosoma japonicum, the soluble immature egg antigen (SIEA) and the SIEA26 28kDa components from the early embryo stages eggs were purified through an AcA column and used to immunize BALB/c mice. The mice divided into three groups were immunized with SIEA, SIEA26 28kDa and immunoadjuvant respectively followed by a schistosome challege infection. The worm burden, the differential count of eggs in the host tissues and the schistosomal uterus were performed 46 days after the infection. The result showed that the decrease rates of intrauterine eggs of the female worms were 54.8% after immunization with SIEA26 28kDa, with a statistical difference being highly significant comparing with the control group. The numbers of schistosome eggs in the animal liver and intestine tissues decreased by 48.07% and 77.41% , respectively. To a more remarkable extent, the numbers of the mature eggs in the liver and intestine dropped by 83.6% and 93.3%, respectively, but most remarkably, the fecal egg decrease rate went up to 87.26%. No similar results were observed in the controls. In conclusion, SIEA26 28kDa can be regarded as acandidate antigen used for the induction of anti embryonation and anti fecundity immunity.