汉坦病毒系统发生分析及其基因分型的研究

目的寻找适于汉坦病毒进行系统发生分析的系统发生树构建方法及其基因片段。选用GenBank中部分有代表性的汉坦病毒株，用M、S、M＋S全序列。以及G1、G2的2003—2302、2741—2950核苷酸片段分别以邻位相连法（NJ）和最大简约法（MP）构建系统发生树。用M＋S、M、G1、以及G2的2003—2302片段以NJ法构建的系统发生树将所选25株病毒分为5个分支，分别对应于HTN、SEO、DOB、PUU血清型及引起HPS的Sin Nomhre血清族。但用G2区的2741—2950片段构建的系统发生树将DOB型与HTN型分为一支。以MP法用M、M＋S及G1片段构建的发生树，结果与NJ法相同。但用S与G2的2003—2302、2741—2950片段构建的系统发生树则不能将所有的病毒株分在相应的血清型。因此，汉坦病毒的基因分型及系统发生分析时，NJ法优于MP法；用M片段的G1核苷酸序列可以替代M-9S片段全序列进行基因分型及系统发生分析。

The genotypes and phylogenetic analysis of Hantavirus

To search for a better method and suitable nucleotide segment for the phylogenetic analysis of Hantavirus, the representative sequence of Hantavirus from GenBank was selected and the phylogenetic trees were constructed with M, S, M+S, G1 and G2 by using either the neighbor-joining (NJ) or maximum parsimony (MP) method. All the strains of Hantavirus could be divided into 5 clades with 3 phylogenetic trees of M, M+S and G1 sequence or segment of G2 (2003-2302 nt) sequence by NJ method, and identified to be HTN, SEO, DOB, PUU serotypes and Sin Nombre virus group respectively. However, the phylogenetic tree constructed with G2 segments (2741-2950 nt) could group HTN and DOB strains into the same clade. With the MP method, the phylogenetic trees of M, M+S segment or segment in G1 were similar to those constructed by NJ method. Yet, the phylogenetic tree of S sequence or G2 (2003-2302 nt or 2741-2950 nt) by MP method could not group all strains into their respective serological groups. These results suggest the NJ method would be a better approach tool for the phylogenetic analysis of Hantavirus than MP method, and segments in G1 provide a reliable alterative to the complete sequence in the phylogenetic analysis.