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电镜检测婴幼儿腹泻标本中晶格状排列与肠道病毒关系的研究
引用本文:刘书锋,徐卫健,关家荣,黄榕,何家鑫 ,杜胜芳.电镜检测婴幼儿腹泻标本中晶格状排列与肠道病毒关系的研究[J].中国人兽共患病杂志,1991(6).
作者姓名:刘书锋  徐卫健  关家荣  黄榕  何家鑫  杜胜芳
作者单位:福建省卫生防疫站,福建省卫生防疫站,福建省卫生防疫站,福建省卫生防疫站
摘    要:本文自1987年至1989年,采用电镜法从1350份5岁以下婴幼儿腹泻粪便标本中检出了24价晶格状排列,检出率为1.78%。通过电镜直接观察及免疫电镜法初步证实了晶格状排列与轮状病毒、腺病毒及冠状病毒结构的关系,明确了晶格是肠道病毒在粪便中蛋白水解酶的作用下其外层亚单位破坏后又自动聚集所形成。

关 键 词:透射电镜  晶格状排列  轮状病毒  腺病毒  冠状病毒

Study on Relationship Between Structure of Enterovirus and Crystalline Patterns Found in Diarrheal Childern' s Fecal Samples Detected by TEM
Liu Shufeng.Study on Relationship Between Structure of Enterovirus and Crystalline Patterns Found in Diarrheal Childern' s Fecal Samples Detected by TEM[J].Chinese Journal of Zoonoses,1991(6).
Authors:Liu Shufeng
Institution:Liu Shufeng
Abstract:We found 24 crystalline patterns from 1350 fecal samples when taking the investigation of en-terovirus in diarrheal fecal samples of childern under five by TEM , the detective rate was 1. 78%. In order to understand the relationship between the crystalline patterns and structure of enterovirus related to diarrhea, we studied the crystalline patterns' formation by TEM observation and IEM. Finally, we confirmed that under the enzymolysis of proteinase in fecal samples, the outlayer of enterovirus could be broken up and then it' s protein subunits formed the crystalline patterns automatically.
Keywords:Transmission electron microscopy  Crystalline pattern Rotavirus  Adenovirus  Coro-navirus  
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