| 结核分枝杆菌异烟肼耐药基因katG的检测 |
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| 引用本文： | 薛欣,邱奕,楚雍烈,赵四海,邵明明,曹春霞.结核分枝杆菌异烟肼耐药基因katG的检测[J].中国病原生物学杂志,2010(7). |
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| 作者姓名： | 薛欣 邱奕 楚雍烈 赵四海 邵明明 曹春霞 |
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| 作者单位： | 西安交通大学医学院病原生物学教研室;西安市结核病医院; |
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| 基金项目： | 西安市科技创新支撑计划项目(No.YF07158) |
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| 摘 要： | 目的探讨结核分枝杆菌异烟肼(INH)耐药的分子机制,建立快速检测结核分枝杆菌INH耐药性的方法。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测105株结核分枝杆菌临床分离株的katG基因,运用DNA测序和生物信息分析比对进行验证。结果以H37Rv标准株为对照,34株敏感株中5株(14.7%)存在KatG基因异常,105株耐药株中55株(52.4%)存在KatG基因异常。结论结核分枝杆菌对INH耐药与KatG基因突变有关,PCR-RFLP技术可快速、准确地检测结核分枝杆菌对INH的耐药性。
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| 关 键 词： | 分枝杆菌 结核 耐药 异烟肼 基因突变 限制性片段长度多态性 |
Detection of the isoniazid-resistant katG gene of Mycobacterium tuberculosis |
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| XUE Xin,QIU Yi,CHU Yong-lie,ZHAO Si-hai,SHAO Ming-ming,CAO Chun-xia.Detection of the isoniazid-resistant katG gene of Mycobacterium tuberculosis[J].Journal of Pathogen Biology,2010(7). |
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| Authors: | XUE Xin QIU Yi CHU Yong-lie ZHAO Si-hai SHAO Ming-ming CAO Chun-xia |
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| Institution: | XUE Xin1,2,QIU Yi2,CHU Yong-lie1,ZHAO Si-hai1,SHAO Ming-ming1,CAO Chun-xia1 |
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| Abstract: | Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to isoniazid (INH) and to establish a method of rapid detection of katG gene mutations in M.tuberculosis.Methods The katG genes of 105 M.tuberculosis clinical isolates were tested by PCR-RFLP and verified by DNA sequencing and bioinformatics analysis.Results Strain H37Rv was used as a control.In 105 M.tuberculosis clinical isolates,5 of 34 (14.7%) drug-susceptible isolates and 55 of 105 (52.4%) INH-resistant isolates had a k... |
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| Keywords: | Mycobacterium tuberculosis resistance isoniazid gene mutation restriction fragment length polymorphism |
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