肝片吸虫感染对大鼠血清和组织自由基代谢的影响

目的 通过大鼠感染肝片吸虫复制感染模型，研究肝片吸虫感染后血清和组织器官抗氧化功能的动态变化。方法 将60只SD大鼠随机分成感染组（n=30）和对照组（n=30），感染组大鼠1次口服25个囊蚴，对照组不感染，于感染前（0周）和感染后（1、3、5、7、9周）宰杀采集血清、肝、肺、心、肾和脾组织，检测感染后GSH-Px、SOD、CAT活性和MDA含量的变化。结果 SD大鼠感染肝片吸虫后，血清中GSHPx先升高后下降；CAT活性在感染后下降；MDA含量在前5周变化不明显。肝组织的GSH-Px活性变化不明显；SOD活性缓慢下降后又缓慢升高；CAT活性降低；MDA含量开始有所升高，稍后有轻微下降；肾脏的GSH-Px活性先缓慢升高，以后则低于对照组；SOD活性呈现平稳下降的趋势，CAT活性开始升高．随后降至低于对照组，MDA含量开始缓慢下降，以后则上升。心组织的GSH—Px活性开始升高．以后迅速下降；SOD活性逐渐升高，然后又缓慢下降；CAT活性逐渐升高，然后又有所下降；MDA含量感染后有所下降。肺组织中的GSH—Px活性逐渐升高，以后逐渐下降；SOD活性5周后开始急剧下降；CAT活性的变化在整个实验期间，除第7周外，其他各周和对照组相比差异均不显著；MDA含量在感染后开始升高，以后又缓慢下降。脾组织中GSH—Px和SOD活性下降；试验组CAT活性先下降，然后升高；MDA含量在前3周变化不明显，基本上处于同一水平，随后缓慢下降，且在第7、9周与对照组差异极显著。结论 自由基参与了肝片吸虫病的发病过程，肝片吸虫感染后机体的器官组织发生了脂质过氧化损伤。

EFFECT OF SD RATS INFECTED WITH FASCIOLA HEPATICA ON FREE RADICALS METABOLISM IN SERUM AND TISSUES

Objective To study the changes of free radicals metabolism in serum and tissues in SD rats post-infection of Fasciola hepatica. Methods SD rats were randomly divided into infection group(n=30) and control group(n=30).The infection group rats were orally dosed with 25 F. hepatica metacercariae. At pre-infection (0 week) and post-infection(1, 3, 5, 7, 9 week), the blood and tissue(liver, kidney, heat, spleen and lung) samples were sampled and determine glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), peroxidase (CAT) and malondialdehyde (MDA). Results The results showed that the serum GSH-Px activity of infection group was firstly increased, then decreased, CAT activity dropped, MDA no significant change in the first 5 weeks. There was no obvious change of liver GHS-Px activity, SOD activity first decreased, then raised, CAT activity dropped. Kidney GHS-Px and CAT activity first increased, then decreased, SOD dropped, MDA level first raised, then decreased. Heart GSH-Px, SOD, CAT first increased, then decreased, MDA decreased post-infection, lung GSH-Px and MDA was first increased, then decreased, SOD dropped and CAT not obviously changed. Spleen GSH-Px and SOD was declined, CAT was first raised, then dropped, MDA not significantly changed in the first 3 weeks, then dropped and there were differences at 7, 9 week between infection and control group. Conclusion It suggested that free radicals participated in the pathogentic course of fascioliasis and there was lipid peroxidation of tissue post-infection.