Foxp3-mRNA表达特征与慢性阻塞性肺疾病患者Thl/Th2免疫应答的调控作用分析

目的分析Foxp3-mRNA表达特征与慢性阻塞性肺疾病(COPD)患者Th1/Th2免疫应答的调控作用,为COPD患者机体免疫状态的监测及其相关机制提供理论基础。方法采用定群抽样的方法选取2016年3月至2017年12月海南省第三人民医院呼吸科接诊并在该院接受后续治疗的COPD患者95例,同时选可比对照85例作为本研究的受试对象;采用PCR法扩增Foxp3-mRNA片段,并采用Western blot检测受试者血清中Foxp3-mRNA表达特征;采用酶联免疫吸附测定法测定Th1/Th2免疫应答系统表达测定的指标:包括干扰素γ(IFN-γ)、白细胞介素2(IL-2)、IL-4、IL-10;所得数据录入SPSS 16.0进行统计学分析。结果COPD病例组的IFN-γ、IL-2、IL-4、IL-10水平均高于对照组且差异均有统计学意义(t=7.282、5.996、7.380、4.835,P值均<0.05),病例组中不同年龄患者的IFN-γ、IL-2、IL-4、IL-10水平差异均有统计学意义(t=22.237、16.943、45.386、31.831,P值均<0.05),病例组中不同病程患者的IFN-γ、IL-2、IL-4、IL-10水平差异均有统计学意义(F=33.226、30.096、29.353、34.300,P值均<0.05)。病例组在592 bp左右位置处可见清晰条带(组1~组5),而对照组(组6)及DNA marker组(组7)基本无条带;Foxp3-mRNA表达量与IFN-γ、IL-2、IL-4、IL-10水平均呈正相关且相关系数均具有统计学意义(r=0.614、0.493、0.529、0.501,P值均<0.05)。结论Foxp3-mRNA在COPD患者中发生特异性表达的理论依据成立,Foxp3-mRNA表达特征与Th1/Th2免疫应答特征存在统计学层面的正相关性,但考虑到我国COPD的流行病学特征,该结论还需要结合COPD患者的环境流行病学因素进一步验证Foxp3-mRNA表达特征与Th1/Th2免疫应答特征在机制层面的联系。

Regulation of Foxp3-mRNA expression and Thl/Th2 immune response in patients with chronic obstructive pulmonary disease

Objective To analyze the expression of Foxp3-mRNA and the regulation of Th1/Th2 immune response in patients with chronic obstructive pulmonary disease(COPD),and to provide a theoretical basis for the monitoring of immune status and its related mechanisms in patients with COPD.Methods A group sampling method was used to select 95 cases of patients with COPD who received a follow-up treatment in a third-grade hospital in the same hospital and 85 controls were conducted at the same time,from March 2016 to December 2017.The Foxp3-mRNA fragment was amplified by PCR and the expression of Foxp3-mRNA in serum was detected by Western blot.The expression of Th1/Th2 immune response system expression assay(including IFN-γ,IL-2,IL-4,IL-10),the obtained data were entered into SPSS 16.0 for statistical analysis.Results The level of IFN-γ,IL-2,IL-4,IL-10 in COPD group were higher than those of in the control group and there were statistical differences(t=7.282,5.996,7.380,4.835,all P<0.05).In the case group,patients of different ages in IFN-γ,IL-2,IL-4,IL-10,were statistically different(t=22.237,16.943,45.386,31.831,all P<0.05).In the case group,patients of different disease durations in IFN-γ,IL-2,IL-4,IL-10 were statistically different(F=33.226,30.096,29.353,34.300,all P<0.05).In the case group,clear bands were seen at about 592 bp(group 1 to group 5),while the control group(group 6)and the DNA marker group(group 7)had no bands.The levels of IL-2,IL-4 and IL-10 were positively correlated and the correlation coefficients were statistically significant(r=0.614,0.493,0.529,0.501,all P<0.05).Conclusions The theoretical basis for the specific expression of Foxp3-mRNA in patients with COPD is established.There is a statistically positive correlation between the expression of Foxp3-mRNA and the characteristics of Th1/Th2 immune response,but considering the epidemic of COPD in China.Characteristics,this conclusion also needs to be combined with the environmental epidemiological factors of patients with COPD to further verify the relationship between Foxp3-mRNA expression characteristics and Th1/Th2 immune response characteristics at the mechanism level.