被动吸烟对大鼠血清肺表面活性蛋白A、D表达的影响

目的 通过烟熏法建立大鼠被动吸烟模型,观察被动吸烟和戒烟对血清肺表面活性蛋白A、D的影响,探讨被动吸烟肺损伤的发生机制.方法 选择健康雄性Wistar大鼠50只,随机分为5组,分别为正常对照组、被动吸烟4周组、被动吸烟8周组、被动吸烟12周组和戒烟组.HE染色观察肺组织的病理改变,采用酶联免疫吸附测定(ELISA)方法检测血清肺表面活性蛋白A、D含量.结果 被动吸烟可导致肺组织炎症反应和肺气肿改变;被动吸烟组和戒烟组大鼠血清肺表面活性蛋白A、D含量较正常对照组升高,随着被动吸烟时间的延长,血清肺表面活性蛋白A、D含量逐渐增加;戒烟组较被动吸烟12周组血清肺表面活性蛋白A、D含量降低.结论 被动吸烟可引起血清肺表面活性蛋白A、D表达增加,提示血清肺表面活性蛋白A、D可能参与了被动吸烟导致肺损伤的发病过程.

Effect of passive smoking on the expression of surfactant protein A and D in rats

Objective Establishment of rat model of passive smoking by smoking,to observe the effect of passive smoking on surfactant protein A and D in rats,to explore the mechanism of immune injury in lung injury by passive smoking.Methods Fifty male wistar rats were randomly divided into five groups:normal control group,passive smoking 4-week group,passive smoking 8-week group,passive smoking 12-week group and cessive smoking group.Observed the pathomorphism changes of lung tissue by Hematoxylinand eosin staining (HE) staining.Detected the the levels of surfactant protein A and D (SP-A、SP-D) in the serum.Results passive smoking can lead to the response of lung inflammatory.The levels of SP-A、D in the serum of rats in passive smoking groups were significantly higher than those in normal control group.Conclusions The expression of SP-A、D significantly increased in the serum of rats with passive smoking.SP-A、D may be implicated in the pathogenesis of lung injury caused by passive smoking.