老年慢性阻塞性肺疾病患者外周血T细胞表达趋化因子受体3的研究

目的 探讨趋化因子受体3(CXCR3)在老年慢性阻塞性肺疾病(COPD)发病中的作用.方法 研究对象均为65岁以上的老年人,分为COPD急性加重期组(20例)、COPD稳定期组(17例)及对照组(14例),分离外周血单个核细胞,流式细胞仪检测CD4+和CD8+T细胞分别占总T细胞的百分比,CD4+CXCR3+T细胞占总CD4+T细胞的百分比,CD8+CXCR3+T细胞占总CD8+T细胞的百分比,以及CD4+CXCR3+和CD8+CXCR3+T细胞上CXCR3的平均荧光强度(MFI).结果 ①三组间及COPD急性加重期组治疗前后CD4+、CD8+T细胞分别占总T细胞的百分比,差异无统计学意义.②CD8+CXCR3+T细胞占总CD8+T细胞的百分比及CD4+CXCR3+T细胞占总CD4+T细胞百分比:COPD急性加重期组较COPD稳定组及对照组明显降低,COPD稳定组较对照组明显升高,差异有统计学意义,P＜0.05.CD4+CXCR3+和CD8+CXCR3+T细胞上CXCR3的MFI三组间差异无统计学意义.③COPD急性加重期组经治疗后CD8+CXCR3+T细胞占总CD8+T细胞的百分比及CD4+CXCR3+T细胞占总CD4+T细胞百分比,CD4+CXCR3+和CD8+CXCR3+T细胞上CXCR3的MFI,均明显降低,差异有统计学意义,P＜0.05.结论 COPD稳定期T细胞上CXCR3的过度表达在COPD慢性炎症过程中起作用.

Expression of CXCR3 on peripheral blood T cell in elderly patients with chronic obstructive pulmonary disease

Objective To investigate the contribution of chemokine receptor CXCR3 in elderly patients with chronic obstructive pulmonary disease(COPD). Methods All subjects were older than 65 years,including 20 patients with acute exacerbation of COPD, 17 patients with stable COPD and 14 healthy controls. Samples of peripheral blood were collected, and mononuclear cells were isolated by density gradient centrifugation. Flow cytometry was applied to measure the percentages of CD4+ and CD8+ cells in total T cells,CD4+ CXCR3+ cells in the total CD4+ cells,CD8+ CXCR3+ cells in the total CD8+ cells,as well as detect the mean fluorescence intensity (MFI) of CXCR3 on CD4+ and CD8+ cells from the three groups. Results ①There were no detectable differences of the percentage of CD4+ and CD8+ cells in total T cells in these groups,the same result was found after the treatment of AECOPD. ②The percentage of T lymphocytes expressing CXCR3 was lower in subjects with acute exacerbation of COPD than stable COPD and healthy controls( P ＜0.05) ,higher level of lymphocytes expressing CXCR3 was observed in stable COPD than controls( P ＜0.05). The MFI of CXCR3 on CD4+ 、CD8+ cells showed no statistical significance in these groups. ③After anti-inflammatory treatment,CD4+ CXCR3+ cells in the total CD4+ cells,CD8+ CXCR3+ cells in the total CD8+ cells and the MFI of CXCR3 on CD4+ 、CD8+ cells markedly decreased in the elderly patients with AECOPD( P ＜0. 05). Conclusions The excessive expression of CXCR3 on T lymphocyte has an effect on the chronic inflammation process of stable COPD.