呼出气冷凝液技术用于检测抗菌药物在呼吸道分布的可行性研究

目的 研究呼出气冷凝液(EBC)技术用于呼吸道表面液体层采样以检测抗菌药物在其中分布的可行性.方法 6名健康受试者单剂空腹口服左氧氟沙星片剂500 mg后,分别于给药前和给药后1、2、4、8、12、24 h收集EBC,以高效液相-荧光检测法测定其中的药物浓度.结果 6名受试者单剂口服左氧氟沙星后在各时间点的中位浓度(最...

Feasibility of exhaled breath condensate on measuring antibiotic distribution in airway surface liquid

Objective To investigate the feasibility of exhaled breath condensate (EBC) as a sampling technique to evaluate the antibiotic distribution in the airway surface liquid. Methods Six healthy volunteers were enrolled. Before and after 1,2,4,8, 12, and 24 hours of orally taking 500 mg levofloxacin, EBC was collected and the level of levofloxacin was measured by high performance liquid chromatography-fluorescence detection. Results The drug concentration in EBC at each time point after oral administration was 0. 000 (0.000, 0.000), 0.000 (0.000, 0.000), 0.000 (0.000,0.230), 0.000 (0. 000,0. 478), 0. 000 (0. 000,0.173), and 0. 015 (0. 000,0. 095) mg/L. Levofloxacin was undetectable in three of all the six subjects, and that was detectable in the other three subjects after four hours of taking levofloxacin,but there was no dynamic trend of drug concentration with time. No adverse effect was observed during the experiment. Conclusions EBC technology is simple and safe for sampling in airway surface liquid, but the consideration of dilution and low levels of drug concentration preclude EBC to become a promising sampling technique on studying the antibiotic distribution in airway surface liquid.