人重组胰岛素样生长因子1对慢性阻塞性肺疾病大鼠膈肌细胞凋亡的保护作用及机制探讨

Objective To investigate the prevetive effect and mechanism of rhIGF-1 on apoptosis of diaphgramatic muscle cell in rats with chronic obstructive pulmonary disease(COPD).Methods Forty-five male Wistar rats were randomly divided into control group(group A),COPD model group(group B),COPD model+rhIGF-1 intervention group(group C),15 in each group.Five rats in each group were killed respectively at a time on the first day,14th,28th day.Take complete diaphragms to detect rate of apoptosis with TUNEL method,detect Fas protein expression with immunohistochemistry and detect Fas gene expression with RT-PCR after weighing.Results Twenty-eight days later,the mass of diaphragms of group B and C was lighter than that of group A(P＜0.05),group C heavier than group B(P＜0.05).At the same time point,rate of apoptosis,Fas protein expression and Fas gene expression of group B and C were higher than those of group A,group C was lower than group B(P＜0.05).Conclusions Fas/FasL mediated apoptosis way involves in the diaphragm apoptosis,rhIGF-1 may interfere with Fas/FasL way to reduce the apoptosis of the diaphragm and may have a protective effect of diaphragm apoptosis.

Preventive effect of rhIGF-1 on diaphgramatic muscle cell apoptosis in rats with chronic obstructive pulmonary disease and mechanism