卒中后抑郁大鼠前额叶皮质、海马和杏仁核胶质纤维酸性蛋白表达

目的：探讨卒中后抑郁（ post-stroke depression, PSD ）大鼠前额叶皮质、海马和杏仁核中胶质纤维酸性蛋白（glial fibrilary acidic protein, GFAP）表达。方法健康成年SD大鼠随机分为正常组、抑郁组、卒中组和PSD组，每组5只。卒中组采用线栓法建立局灶性脑缺血模型；抑郁组采用慢性不可预见性温和应激（chronic unpredictable mild stress, CUMS ）结合孤养建立大鼠慢性应激抑郁模型；PSD组采用线栓法建立局灶性脑缺血模型，术后1周加以CUMS 和孤养建立PSD 大鼠模型。在首次CUMS 后第1天、第8天、第15天和第29天进行蔗糖水消耗实验（ sucrose preference test, SPT）和旷场实验（open-field test, OFT）评价抑郁行为，在第29天应用免疫荧光染色法检测前额叶皮质、海马和杏仁核GFAP表达。结果在CUMS后第29天时，抑郁组和PSD组SPT 蔗糖水消耗量以及OFT水平和垂直运动评分均显著低于正常组和卒中组（P均＜0.05）；PSD组前额叶皮质、海马和杏仁核GFAP免疫阳性细胞数量均显著少于正常组、抑郁组和卒中组（P均＜0.05），而正常组、抑郁组和卒中组之间差异均无统计学意义（ P均>0.05）。结论 PS D 大鼠前额叶皮质、海马和杏仁核GFAP表达下降可能在PSD发病过程中发挥着一定的作用。

Expressions of glial fibrillary acidic protein in the prefrontal cortex,hippocampus, and amygdala in post-stroke depression in rats

Objective To investigate the expressions of glial fibrilary acidic protein (GFAP) in the prefrontal cortex, hippocampus and amygdala in post-stroke depression (PSD) in rats. Methods Healthy adult SD rats w ere randomly divided into a normal group, a depression group, a stroke group, and a PSD group ( n=5 in each group). A model of focal cerebral ischemia w as induced by the intraluminal suture method in the stroke group;a rat chronic stress depression model was induced by using chronic unpredicted mild stress (CUMS) combined w ith single housing in the depression group;a model of focal cerebral ischemia w as induced by the intraluminal suture method in the PSD group. A rat PSD model w as induced by CUMS and single housing at 1 week after operation. The sucrose preference test (SPT) was performed in each group at day 1, 8, 15, and 29 after the first CUMS, and the open-field test ( OFT ) w as used to evaluate the depressive behaviors. Immunofluorescence staining was used to detect the expression of GFAP in the prefrontal cortex, and hippocampus and amygdala at day 29. Results At day 29 after CUMS, the sucrose solution consumption in SPT and the scores of horizontaland vertical movement in OFT in the depression group and PSD group w ere decreased significantly compared w ith the normal group and the stroke group (al P<0.05);the numbers of GFAP immunopositive cel s in the prefrontal cortex, hippocampus, and amygdala in the PSD group w ere significantly less than those in the normal group, depression group and stroke group (al P<0.05), and there w ere no significant differences among the normal group, depression group and stroke group (al P>0.05). Conclusion The decreased expression of GFAP in the prefrontal cortex, hippocampus, and amygdale may play a certain role in the process of PSD.