Isolation and characterization of Marine fungal metabolites against clinical pathogens

ObjectiveTo isolate and identify of marine fungal metabolites against clinical bacterial pathogens. To optimize the production medium for isolated fungus.MethodMarine fungus isolated from water and sediment samples from different places of Sundarbans mangrove, Muttukadu (Chennai) and Parangipettai in India. Antimicrobial substance from marine fungi was produced by agar plate method. The potent fungal were inoculated on production medium and extracted was done. The extracted compound was checked for anti bacterial activity. Suitable production medium were optimized.ResultTotally 30 fungal isolates were recovered and morphologically 10 different strains were belongs to the fungal genera such as Fusarium, Aspergillus, Mucor and Penicillium. Preliminary screening results showed 3 fungal isolates showed promising activity. After production of potent fungal SS2 crude extracts showed highest inhibition against the bacterial pathogens out of 3 fungal isolates. The results showed maximum zone in 20mm against E. coli and minimum 10 mm against Vibrio sp.ConclusionsThe present study identified Fusarium sp isolated from Sundarbans mangrove water as a potential source for bioactive compounds. Further isolation of active compound from potential fungal isolates will leads to the discovery of effective antimicrobials.