| 用PCR方法检测粪便难辨梭状芽胞杆菌 |
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| 引用本文： | 关宏,钱家鸣,陆星华,杨晓鸥.用PCR方法检测粪便难辨梭状芽胞杆菌[J].临床消化病杂志,2001,13(4):147-148. |
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| 作者姓名： | 关宏 钱家鸣 陆星华 杨晓鸥 |
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| 作者单位： | 中国医学科学院中国协和医科大学北京协和医院消化科, |
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| 摘 要： | 目的：采用PCR方法检测粪便难辨梭状芽胞杆菌。方法：北京协和医院的36例应用抗生素后腹泻的病人粪便标本，采用PCR方法对难辨梭状芽胞杆菌（以下简称C．D）的毒素B基因和毒素A基因进行扩增，同时进行厌氧细菌培养。结果：PCR法毒素B基因的阳性率为46．7％（16／36），毒素A基因为36．1％（13／36）；用厌氧菌培养的阳性率为19．2％（7／36）。PCR检测C．D毒素基因方法与厌氧培养法的一致性为100％。结论：用PCR方法检测C．D比厌氧培养法检出率明显增高（P＜0．05），与厌氧菌培养法的一致性高。
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| 关 键 词： | 难辨梭状芽胞杆菌 毒素A 毒素B PCR 检测 |
| 修稿时间： | 2001年3月1日 |
Use of the Polymerase Chain Reaction for the Detection of Clostridium Difficile in Human Feces |
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| Guan Hong,Qian Jia ming,Lu Xing hue,et al.Use of the Polymerase Chain Reaction for the Detection of Clostridium Difficile in Human Feces[J].Chinese Journal of Clinical Gastroenterology,2001,13(4):147-148. |
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| Authors: | Guan Hong Qian Jia ming Lu Xing hue |
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| Institution: | Guan Hong,Qian Jia ming,Lu Xing hue,et al Department of Gastroenterology,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730 |
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| Abstract: | Purpose: Use of the PCR for the detection of clostridium difficile(C.D) in human feces. Methods: 36 patients with typical clinical manifestation of C.D colitis from the Peking Union Medical College Hospital were chosen for this study. PCR was used for detection of toxin A & B of C.D in stool specimens, while the cultures for C.D were performed under anaerobic conditions. Results: 46.7%(16/30) stool specimens were positive for toxin B, 36.1%(13/36) were positive for toxin A, 19.2%(7/36) were positive for anaerobic culture. The consistency between the method of PCR and anaerobic culture was 100%. Conclusion: The method of PCR for detection of C.D in human feces is more sensitive than of the culture (P<0.05) and there is high consistency with the anaerobic culture. |
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| Keywords: | Clostridium difficile Toxin A Toxin B PCR |
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