冠状动脉介入患者围手术期血清Rho激酶-1变化分析

目的评价接受冠状动脉介入术（PCI）患者血清Rho激酶-1（ROCK-1）围术期水平的变化情况。方法纳入接受PCI的患者84例,其中急诊PCI组28例,择期PCI组56例;另纳入有不典型心绞痛症状但冠脉造影正常者24例作为对照组。采用酶联免疫吸附法（ELISA）分别测定各组PCI术前、PCI术后4h/1d/4d患者血清ROCK-1、hs-CRP和TNF-α水平。结果急诊PCI组患者术前血清ROCK-1、hs-CRP和TNF-α表达均高于择期PCI组和对照组,差异有统计学意义（P〈0.05）;择期PCI组患者术前血清ROCK-1、hs-CRP和TNF-α表达浓度高于对照组,差异有统计学意义（P〈0.05）。急诊PCI组和择期PCI组患者在接受PCI手术治疗后的三种炎性因子表达均增加,以急诊PCI更为明显,但术后1d急诊PCI组的炎症因子表达即明显回落,水平低于择期PCI组。对照组在冠脉造影前后三种炎症因子表达无统计学差异（P〉0.05）。结论介入治疗可导致机体ROCK-1、hs-CRP和TNF-α水平一过性升高,且病变范围越广则炎症反应水平越高。

The expressions of Rho kinase-1 in perioperative period of the patients with percutaneous coronary intervention

Objective To review the expressions of serum Rho kinase（ROCK-1）in patients with coronary heart disease（CHD）during perioperative period of percutaneous coronary intervention（PCI）.Methods CHD patients（n=84） accepted PCI were selected,among them 28 was in emergency PCI group,and 56 in selective PCI group.In addition,other 24 patients with atypical angina pectoris but normal coronary angiography（CAG）were selected as control group.The expressions of serum ROCK-1,hs-CRP and TNFα were detected by using enzyme-linked immunosorbent assay（ELISA）before PCI and 4 hours,one day and 4 days after PCI.Results Before PCI,the expressions of ROCK-1,hs-CRP and TNFα were higher in emergency PCI group than those in other two groups（P 0.05）,and were higher in selective PCI group than those in normal control group（P 0.05）.After PCI,the expressions of ROCK-1,hs-CRP and TNFα increased in emergency PCI group and selective PCI group,and more significantly in emergency PCI group.These expressions fell back in emergency PCI group one day after PCI,and were lower than those in selective PCI group.The expressions of ROCK-1,hsCRP and TNFα had no difference in normal control group before and after CAG.In selective PCI group,the expressions of ROCK-1,hs-CRP and TNFα increased with the increase of disease vessels.Conclusion The expressions of ROCK-1,hs-CRP and TNFα can be induced to transient increase by PCI,and the levels of inflammatory reactions will increase with the extension of PCI.