干扰LINC00707对食管癌细胞生物行为的影响

目的探讨干扰LINC00707对食管癌细胞生物行为的影响及分子机制。方法选取51例食管癌患者癌组织及癌旁正常组织,用实时荧光定量-聚合酶链反应(RT-qPCR)检测LINC00707和miR-382-5p的表达水平;将食管癌细胞EC9706随机分为对照(con)组、si-LINC00707组、si-NC组、miR-382-5p组、miR-NC组、anti-miR-382-5p组、anti-miR-NC组、si-LINC00707+anti-miR-382-5p组;用四甲基偶氮唑盐(MTT)比色法检测细胞活性;克隆形成实验检测细胞克隆形成数;流式细胞术检测细胞凋亡率;划痕实验检测细胞迁移距离;Western印迹法检测蛋白表达;双荧光素酶报告实验检测LINC00707和miR-382-5p的靶向关系。结果与癌旁正常组织相比,食管癌组织中LINC00707表达水平升高,miR-382-5p表达水平降低,差异有统计学意义(P<0.05);且LINC00707和miR-382-5p表达水平呈负相关(P<0.05)。干扰LINC00707或过表达miR-382-5p后,EC9706细胞活性降低,克隆形成数减少,细胞凋亡率升高,细胞迁移距离缩短,Bax表达水平升高,Bcl-2表达水平降低,差异有统计学意义(P<0.05)。抑制miR-382-5p表达与过表达miR-382-5p对EC9706细胞的作用相反。且抑制miR-382-5p表达逆转了干扰LINC00707对EC9706细胞的作用。LINC00707靶向调控miR-382-5p。结论干扰LINC00707可能通过上调miR-382-5p抑制食管癌细胞的恶性生物学行为。

Effect of interference with LINC00707 on the biological behavior of esophageal cancer cells

Objective To explore the effect of interference with LINC00707 on the biological behavior of esophageal cancer cells and its molecular mechanism.Methods 51 cases of esophageal cancer patients with cancer tissues and adjacent normal tissues were selected,and real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression levels of LINC00707 and miR-382-5p;esophageal cancercells EC9706 were randomly divided into control(con)group,si-LINC00707 group,si-NC group,miR-382-5p group,miR-NC group,anti-miR-382-5p group,anti-miR-NC group,si-LINC00707+anti-miR-382-5p group;tetramethylazolium salt colorimetric method(MTT)was used to detect cell viability;clone formation test was used to detect the number of cell clones;flow cytometry was used to detect cell apoptosis rate;scratch experiment deteced cell migration distance;Western blot method was used to detect protein expression;dual luciferase reporter experiment was used to detect the targeting relationship between LINC00707 and miR-382-5p.Results Compared with normal tissues adjacent to cancer,the expression level of LINC00707 in esophageal cancer tissues was increased,and the expression level of miR-382-5p was decreased;and the expression levels of LINC00707 and miR-382-5p were negatively correlated(P<0.05).After interference with LINC00707 or overexpression of miR-382-5p,EC9706 cell viability was decreased,the number of clones formed was decreased,the apoptosis rate was increased,the cell migration distance was shortened,the expression level of Bax was increased,and the expression level of Bcl-2 was decreased(P<0.05).Inhibition of miR-382-5p expression was opposite to the effect of overexpression of miR-382-5p on EC9706 cells.And inhibiting the expression of miR-382-5p reversed the effect of interfering with LINC00707 on EC9706 cells.LINC00707 targeted miR-382-5p.Conclusions Interference with LINC00707 may inhibit the malignant biological behavior of esophageal cancer cells by up-regulating miR-382-5p.