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| 补肾中药对核受体共调节因子表达的影响 | |
| 引用本文： | 张磊,郑腾,杨民和,张志灯,蔡晶.补肾中药对核受体共调节因子表达的影响[J].中国老年学杂志,2010,30(4). |
| 作者姓名： | 张磊 郑腾 杨民和 张志灯 蔡晶 |
| 作者单位： | 1. 福建师范大学,福建,福州,350108 2. 福建出入境检验检疫局 3. 福建中医学院中西医结合研究院 |
| 基金项目： | 国家自然科学基金，福建省中西医结合老年性疾病重点实验室开放课题/陈可冀中西医结合发展基金资助 |
| 摘 要： | 目的 通过检测含药血清培养的人乳腺癌细胞中核受体共激活因子(steroid receptor coactivator-1,SRC-1)和核受体共抑制因子(nuclear receptor corepressor,NCOR)的mRNA表达,从分子水平上探讨补肾中药对核受体共调节因子表达的影响.方法 Wistar大鼠随机分为3个大组,每大组再分雌雄2组,每大组分别用淫羊藿、女贞子、蒸馏水灌胃14 d,制备含药血清,并用含药血清培养雌激素受体缺失的乳腺癌细胞MDA-MB-231,利用实时荧光定量RT-PCR方法检测SRC-1和NCOR的mRNA表达.结果 补肾阳中药淫羊藿雄性大鼠含药血清上调了SRC-1的表达,对NCOR没有显著影响;而补肾阴中药女贞子下调了SRC-1共激活因子的表达,上调了NCOR的表达.结论 补肾中药淫羊藿、女贞子对核受体共调节因子的表达有调节作用. |
| 关 键 词： | 核受体共激活因子 核受体共抑制因子 淫羊藿 女贞子 |
Effect of traditional and herbal drugs of tonifying kidney on expression of nuclear receptor coregulator |
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| ZHANG Lei,ZHENG Teng,YANG Min-He,et al..Effect of traditional and herbal drugs of tonifying kidney on expression of nuclear receptor coregulator[J].Chinese Journal of Gerontology,2010,30(4). | |
| Authors: | ZHANG Lei ZHENG Teng YANG Min-He |
| Institution: | ZHANG Lei,ZHENG Teng,YANG Min-He,et al.Fujian Normal University,Fuzhou 350108,Fujian,China |
| Abstract: | Objective To explore the effect of different tonifying kidney on expressions of steroid receptor coactivator-1 (SRC-1) and nuclear receptor repressor (NCOR) mRNA. Methods Wistar rats were divided into 3 groups and each group was divided into male and female subgroups. Wistar rats were intragastric administration with epimedium,fructus ligustri lucidi and distilled water for 14 days to make drug serum. Human breast carcinoma cell (MDA-MB-231) was cultured with drug serum and the expressions of SRC-1 and NCOR... |
| Keywords: | Steroid receptor coactivator Nuclear receptor repressor Epimedium Fructus ligustri lucidi |
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