从细胞外信号调节激酶-1mRNA在肝纤维化大鼠肝组织中的表达来探讨姜黄素的治疗作用

目的:探讨ERK-1mRNA在CCl4致实验性肝纤维化大鼠肝组织中的表达及姜黄素(curcumin)对它们的影响。方法:建立CCl4诱导的大鼠实验性肝纤维化的模型,用免疫组化法比较正常组、模型组以及姜黄素组大鼠的α-平滑肌肌动蛋白(α-smooth muscle action,α-SMA)的表达,用RT-PCR法比较各组大鼠肝组织胞外信号调节激酶-1(Extracellular-regulated kinase-1,ERK-1)的表达。结果:模型组大鼠α-SMA以及ERK-1mRNA的表达增高明显,而姜黄素组则较模型组低。结论:姜黄素可能通过抑制α-SMA的表达,抑制ERK-1的促肝星状细胞(hepatic stellate cell,HSC)的增殖作用,从而产生抗四氯化碳诱导的大鼠肝纤维化的作用。

Expression of ERK-1mRNA in rat with liver fibrosis and the influence of curcumin

Objective:To evaluate Expression of ERK-1mRNA in rat with liver fibrosis and the influence of curcumin to them.Methods:Liver fibrosis in rats were induced by carbon tetrachloride(CCl4).Rats were divided into three groups:normal control group,CCl4 model group and curcumin group.The expression of alpha-Smooth Muscle Action were detected by immunohistochemistry.The mRNA expression of extracellular signal-regulated kinase(ERK-1)were determined by RT-PCR.Results:The expression of alpha-Smooth Muscle Action were significantly lower in the rats treated with curcumin compared with those of the model group(P<0.05).The mRNA expression of ERK-1 were significantly higher in model group,but obviously lower in curcumin treated group(P<0.05).Conclusion:curcumin is able to inhibit the activiation of HSC through suppress ing the expression of alpha-Smooth Muscle Action and it also can inhibit the proliferation of HSC through suppressing the mRNA expression of ERK-1.