姜黄素对肺炎支原体感染肺炎幼鼠抗炎作用及机制初步探讨

目的基于NF-κB途径探讨姜黄素对肺炎支原体感染肺炎幼鼠抗炎作用及机制初步探讨。方法选取50只SPF级BALB/c小鼠随机分为五组,分别为空白对照组(A组)、模型组(B组)、姜黄素高剂量组(C组),姜黄素中剂量组(D组)和姜黄素低剂量组(E组),模型组及姜黄素治疗组采用肺炎支原体标准株滴鼻法进行建模,HE染色观察肺脏病理组织学变化;免疫组化染色测定肺组织中NF-κB p65阳性细胞的积分光密度;采用Western blot实验检测肺组织中NF-κB p65蛋白表达变化;使用ELISA法检测IL-6、IL-8和TNF-α水平。结果模型组小鼠肺组织病理改变明显,姜黄素高剂量组小鼠肺组织与模型组相比,小鼠肺泡结构比较完整,肺泡与气管结构清晰,炎细胞明显减少,姜黄素中剂量组对小鼠有一定的治疗效果,姜黄素低剂量组小鼠和模型组小鼠比较并无明显差异;与空白对照组相比,模型组小鼠肺组织中NF-κB p65的IOD值、NF-κB p65蛋白表达和支气管灌洗液中IL-6、IL-8和TNF-α表达明显升高(P<0.01),与模型组小鼠相比,姜黄素高剂量组和姜黄素中剂量组小鼠肺组织中NF-κB p65的IOD值、NF-κB p65蛋白表达和支气管灌洗液中IL-6、IL-8和TNF-α表达明显降低(P<0.01)。与模型组小鼠相比,姜黄素低剂量组与模型组小鼠肺组织中NF-κB p65的IOD值、NF-κB p65蛋白表达和支气管灌洗液中IL-6、IL-8和TNF-α表达相比差异无统计学意义(P>0.05)。结论姜黄素可改善肺炎支原体感染幼鼠症状且能够抑制炎症因子,其机制可能是通过抑制NF-κB通路的激活,表明姜黄素可作为治疗肺炎支原体感染的潜在药物。

A preliminary study on anti-inflammatory effect and mechanism of curcumin on young mice infected with Mycoplasma pneumoniae pneumonia

Objective To investigate the anti-inflammatory effect and mechanism of curcumin on young mice infected with Mycoplasma pneumoniae pneumonia based on NF-κB pathway.Methods 50 SPF BALB/c mice were randomly divided into five groups,including the blank control group(the group A),the model group(the group B),the high-dose curcumin group(the group C),the curcumin middle-dose group(the D group)and the curcumin low-dose group(the E group).The model group and the curcumin treatment group were modeled by the standard strain of Mycoplasma pneumoniae,and HE staining was used to observe histopathological changes of lung tissue.The integrated optical density of NF-κB p65 positive cells in lung tissue were determined by immunohistochemical staining,the NF-κB p65 expression in lung tissue was detected by Western blot experiment,and the levels of IL-6,IL-8 and TNF-αwere detected by ELISA.Results The pathological changes of the lung tissue of the mice in the model were obvious.Compared with the high-dose curcumin group,the structure of the mouse alveoli was relatively complete,the structure of the alveoli and trachea was clear,and the inflammatory cells were significantly reduced.The middle-dose curcumin group had a certain therapeutic effect on mice.There was no significant difference between the low-dose curcumin group and the model group.Compared with the blank control group,the value of NF-κB p65 IOD,the expression of NF-κB p65 protein,and the levels of IL-6,IL-8 and TNF-αin bronchial lavage fluid were significantly increased in the model group(P<0.01).Compared with the model group,the value of NF-κB p65 IOD,the expression of NF-κB p65 protein,and the levels of IL-6,IL-8 and TNF-αin bronchial lavage fluid were significantly reduced in the high-dose group and NF-κB p65 the middle-dose group(P<0.01).Compared with the model mice,there was no statistically significant difference in NF-κB p65 IOD,NF-κB p65 protein expression,and IL-6,IL-8 and TNF-αin bronchial lavage fluid in the low-dose curcumin group and the model group(P>0.05).Conclusion Curcumin can improve the symptoms of Mycoplasma pneumoniae infected young mice and inhibit inflammatory factors.The mechanism may be through inhibiting the activation of NF-κB pathway,indicating that curcumin can be used as a potential drug for the treatment of Mycoplasma pneumoniae infection.