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| 中华按蚊热激蛋白40(HSP40)cDNA序列的全长扩增 | |
| 引用本文： | 李云华,朱诗应,孙庆文,朱淮民.中华按蚊热激蛋白40(HSP40)cDNA序列的全长扩增[J].国际医学寄生虫病杂志,2010,37(3). |
| 作者姓名： | 李云华 朱诗应 孙庆文 朱淮民 |
| 作者单位： | 1. 第二军医大学病原生物学教研室,上海,200433 2. 第二军医大学微生物学教研室,上海,200433 3. 第二军医大学数理教研室,上海,200433 |
| 基金项目： | 国家重点基础研究发展规划(973计划)，国家传染病重大专项 |
| 摘 要： | 目的 为获取中华按蚊感染约氏疟原虫后24 h的高表达基因热激蛋白40(HSP40)cDNA的全长序列. 方法 运用抑制性消减杂交技术(suppression substractive hybridization,SSH)构建中华按蚊感染约氏疟原虫后24 h的差异表达基因文库,从高表达文库中获得长度为363 bp的HSP40基因的cDNA片段,运用RACE技术对其cDNA序列进行全长扩增. 结果 获得中华按蚊HSP40 cDNA的全长序列,总长度为1 159 bp,开放阅读框为1 014 bp,编码337个氨基酸. 结论 获得了中华按蚊HSP40 cDNA的全长序列. |
| 关 键 词： | 疟原虫 热激蛋白 差异表达基因 全长扩增 |
RACE amplification of full-length cDNA of HSP40 gene from Plasmodium yoelii infected Anopheles sinensis |
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| LI Yun-hua,ZHU Shi-ying,SUN Qing-wen,ZHU Huai-min.RACE amplification of full-length cDNA of HSP40 gene from Plasmodium yoelii infected Anopheles sinensis[J].International JOurnal of Medical Parasitic Diseases,2010,37(3). | |
| Authors: | LI Yun-hua ZHU Shi-ying SUN Qing-wen ZHU Huai-min |
| Abstract: | Objective To isolate differentially expressed gene HSP40 from Anopheles sinensis induced by infection with Plasmodium yoelii yoelii after 24 h, and to amplify the full-length cDNA of HSP40. Methods A 363 bp of HSP40 cDNA was isolated from the suppression substractive hybridization (SSH) library. The full length of cDNA was amplified using the RACE (rapid-amplification of cDNA ends) technology. Results The full-length cDNA of HSP40 was completely amplified. The whole length was 1 159 bp, with an open reading frame of 1 014 bp, encoding 337 amino acids. Conclusions The full-length cDNA of HSP40 has been completely amplified. |
| Keywords: | Plasmodium Heat shock protein Differentially expressed genes RACE amplification |
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