| 紫杉醇对大鼠血管平滑肌细胞G_1-S检查点作用的研究 |
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| 引用本文： | 静亮,彭希,谢敏杰,喻志源,吕家高,王伟.紫杉醇对大鼠血管平滑肌细胞G_1-S检查点作用的研究[J].内科急危重症杂志,2014(3):193-195. |
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| 作者姓名： | 静亮 彭希 谢敏杰 喻志源 吕家高 王伟 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院,武汉430030 |
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| 基金项目： | 国家自然科学基金资助项目（No：81030021） |
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| 摘 要： | 目的:探讨紫杉醇对大鼠血管平滑肌细胞(VSMC)和血管内皮细胞(VEC)G1-S检查点的作用。方法:将大鼠VSMC与VEC进行原代培养,50 nmol/L紫杉醇干预72 h后进行免疫荧光染色和蛋白印迹法检测。结果:同VEC相比,紫杉醇干预的VSMC中BrdU表达明显增加(均P0.05)。紫杉醇干预的VSMC中cyclin D1、P27表达高于VEC,P21表达较低(均P0.05)。结论:紫杉醇干预的VSMC同VEC相比,在增殖率与细胞周期调节因子表达方面具有明显差异,紫杉醇对VSMC G1-S检查点有特异性调节作用。
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| 关 键 词： | 血管平滑肌细胞 血管内皮细胞 紫杉醇 G1-S检查点 |
Study on the effect of paclitaxel on Gt-S checkpoint of rat vascular smooth muscle cell |
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| JING Liang,PENG Xi,XIE Min-jie,YU Zhi-yuan,Lu Jia-gao,WANG Wei.Study on the effect of paclitaxel on Gt-S checkpoint of rat vascular smooth muscle cell[J].Journal of Internal Intensive Medicine,2014(3):193-195. |
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| Authors: | JING Liang PENG Xi XIE Min-jie YU Zhi-yuan Lu Jia-gao WANG Wei |
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| Institution: | ( Tongji Hospital Affiliated to Tongji Medical College, Huangzhong University of Science and Techonology, Wuhan 430030, China) |
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| Abstract: | Objective： To explore the effects of paclitaxel on G1 -S checkpoint of vascular smooth muscle cell （VSMC） and vascular endothelial cell （VEC） of rats. Methods： VSMC and VEC of rats underwent primary culture and were intervented by 50 nmol/L paclitaxel for 72 hours and then evaluated by immunohistochemistry and western blotting. Results： Compared with VEC, the content of BrdU was increased in paclitaxel-treated VSMC （P 〈 0.05）, paclitaxel-treated VSMC could express more cyclin D1, P27 and fewer P21 （P 〈 0. 05 ）. Conclusions ： Paclitaxel-treated VSMC shows significant differences in the expressions of cell cycle regulators and proliferation rates as compared with those of VEC, thus suggests the specific G1 -S checkpoint regulation effect of paclitaxel on VSMC. |
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| Keywords: | Vascular smooth muscle cell Vascular endothelial cell Paclitaxel G1-S checkpoint |
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