高通量聚砜膜透析器F60对尿毒症晚期患者的治疗效果分析

目的:探讨高通量聚砜膜透析器F60对尿毒症晚期患者疗效及Krüppel样因子2(KLF2) mRNA表达的影响。方法:回顾性选择2015年5月至2018年6月宝鸡市中心医院血液净化中心行维持血液透析的尿毒症晚期患者100例,将其分为2组:实验组采用高通量聚砜膜透析器F60,对照组采用聚砜膜透析器F6,每组50例。测定2组患者透析前、后糖基化终末产物(AGEs)、血清各溶质浓度、KLF2的mRNA表达情况以及腹膜间皮细胞衰老情况。结果:实验组患者透析后AGEs、血清各溶质浓度及腹膜间皮细胞衰老率显著降低,而腹膜组织KLF2的mRNA表达显著增加,且实验组高于对照组(P均0. 05)。KLF2的mRNA表达量与腹膜间皮细胞衰老呈负相关(r=-0. 789,P=0. 021)。结论:尿毒症晚期患者采用高通量聚砜膜透析器F60较聚砜膜透析器F6的透析效果更佳,可能与其可提高KLF2的mRNA表达,降低腹膜间皮细胞衰老有关。

Analysis of therapeutic effect of high throughput polysulfone membrane dialyzer F60 on advanced uremia patients

Objective: To investigate the effect of high-throughput polysulfone membrane dialyzer F60 on the expression of Kruppel-like factor 2 mRNA in patients with advanced uremia. Methods: A total of 100 patients with advanced uremia in Nephrology Department of Baoji Central Hospital from May 2015 to June 2018 were selected and divided into experimental group (high flux polysulfone membrane dialyzer F60) and control group (polysulfone membrane dialyzer F6) (n=50 in each group). Advanced glycation end-products (AGEs), serum solute concentration, mRNA and protein expression of KLF2 and peritoneal mesenchymal cell aging were measured before and after treatment in the two groups. Results: AGEs, serum solute concentration and senescence rate of peritoneal mesenchymal cells were significantly decreased, while mRNA expression of KLF2 was significantly increased in the experimental group after dialysis, and the changes in the experimental group were significantly higher than those in the control group (P all< 0.05).The mRNA expression levels of KLF2 were negatively correlated with the senescence of peritoneal mesenchymal cells(r=-0.789,P=0.021). Conclusion: The dialysis effect of high flux polysulfone membrane dialyzer F60 in late uremia patients was better than that in polysulfone membrane dialyzer F6, which may be related to the increased mRNA expression of KLF2 and the decreased aging of peritoneal mesenchymal cells.