人胎儿肝组织形态学及蛋白质组学分析

目的 探讨不同发育时期胎儿肝组织形态学及蛋白质表达谱的差异.方法 胎儿肝组织常规切片、HE染色、光学显微镜下观察肝组织形态.采用基于双向电泳-基质辅助激光解吸电离飞行时间质谱的蛋白质组学分析方法,分析早,晚期胎儿肝脏蛋白质表达谱的差异,并与肝癌组织蛋白质表达谱比较;用Mascot软件对差异表达的蛋白质在蛋白质数据库中进行检索并鉴定.结果 早、晚期胎儿肝脏不仅组织形态学上存在明显差异,而且蛋白质表达谱也存在明显差异.与肝癌组织蛋白质表达谱比较,对有差异的和相似的32个蛋白质点进行分析,初步鉴定出26个蛋白质.它们涉及细胞的增殖、凋亡及各种物质代谢等方面.结论 提示肝癌的发生是原始胎儿肝细胞发育分化的重现,在肝癌的发生过程中由于癌细胞遗传特性的紊乱获得了某些胎儿肝细胞的特性.

Histomorphology and proteomics analysis of human fetus liver

Objective To investigate the histomorphology and protein expression profiles of human fetal liver at different developmental stages.Methods The protein expression patterns of human fetus livers at early and late stages were profiled by two-dimensional gel electrophoresis(2-DE)combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)analysis.The differentially expressed proteins were identified by searching the protein databases with Mascot or ProFound softwares.Results The obvious differences between early and late fetus liver were not only in morphology but also in protein expression profiles.Compared with protein expression pattern of hepatocel-lular carcinoma,32 differential or similar protein spots were analyzed by MALDI-TOF MS,and 26 proteins,including proteins involved in cell proliferation,apoptosis and metabolism,were identified.Conclusion The data suggest that the hepatic cancer cells regain some characteristics of fetal hepatic cells during theprocess of carcinogenesis.