| 硫化氢对离体大鼠心室肌细胞ATP依赖的钾通道外向电流的影响 |
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| 引用本文： | 李艳兵,钟光珍,杨新春,刘秀兰.硫化氢对离体大鼠心室肌细胞ATP依赖的钾通道外向电流的影响[J].中华心血管病杂志,2000,37(1):445-448. |
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| 作者姓名： | 李艳兵 钟光珍 杨新春 刘秀兰 |
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| 作者单位： | 首都医科大学附属北京朝阳医院心脏中心,100020; |
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| 基金项目： | 国家自然科学基金北京市自然科学基金北京科技新星计划 |
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| 摘 要： | Objective To investigate the effects of endogenous and exogenous hydrogen sulfide (H2S) on the KATP current in isolated rat ventricular myoeytes. Methods Ventrieular myoeytes were isolated from rat heart by modified Langendoff perfusion with collagenase. KATP current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique. Results The density of KATP current was significantly reduced by 200 μmol/L DL-propargylglyeine (PPG, an irreversible inhibitor of the H2S) (5.3258±0.7556) pA/pF vs. (3.7856±0.4312) pA/pF, P ＜ 0.01] in a time-dependent way. The density of KATP current could be significantly increased by NariS(a H2S donor, 9.375, 18.75, 37.5,75, 150 μmol/L) in a concentration-dependent manner (6.6310±0.6092) pA/pF vs. (9.0949±1.0259)pA/pF at 150 μmol/L, P ＜ 0.01]. Conclusion Both endogenous and exogenous H2S could open KATP channels and enhance the KATP current in rat ventricular myocytes.
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| 关 键 词： | 硫化氢 钾通道 膜片钳术 |
Effects of hydrogen sulfide on the KATP current in isolated rat ventricular myocytes |
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| LI Yan-bing,ZHONG Guang-zhen,YANG Xin-chun,LIU Xiu-lan.Effects of hydrogen sulfide on the KATP current in isolated rat ventricular myocytes[J].Chinese Journal of Cardiology,2000,37(1):445-448. |
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| Authors: | LI Yan-bing ZHONG Guang-zhen YANG Xin-chun LIU Xiu-lan |
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| Abstract: | |
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| Keywords: | Hydrogen sulfidePotassium channelsPatch-clamp techniques |
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