细胞间粘附分子—1表达水平对心肌再灌注损伤的影响及N—乙 …

探讨细胞间粘附分子表达与中性粒细胞浸润与心肌血再灌注损伤的关系并观察Ｎ－乙酰半胱氨酸对ＩＲＩ的保护效果。方法大鼠１１６只,分设：（１）缺血再灌注（ＩＲ）组;（２）ＩＲ＋ＮＡＣ组;（３）假手术对照组,并分庙再灌注１、３、６、１２、２４ｈ时相点。取缺血心肌用原位杂交和免疫组织检测ＩＣＡＭ－１ｍＲＮＡ及其蛋白表达水平,用酶法测定ＰＭＮｓ浸润数,ＴＴＣ染色法测心肌梗死范围,结果心肌再灌注时,ＩＣＡＭ－１表

Influence of expression of intercellular adhesion molecule-1 on myocardial ischemic reperfusion injury and cardiac protective effect of N-acetylcysteine

Objective　To investigate the influence of expression of intercellular adhesion molecule-1(ICAM-1) on myocardial ischemic reperfusion injury (IRI), myocardial infiltration of polymorphonuclear neutrophils(PMNs) and infarction size, and the cardiac protective effect of N-acetylcysteine(NAC). Methods　One hundred and sixteen rats were divided into three groups IR, IR+NAC and sham-group. The ischemic myocardial samples were studied at 1,3, 6,12, 24 hours after ischemic reperfusion (IR). The levels of expression of ICAM-1 mRNA were evaluated by in situ hybridization and the protein by immunocytochemistry. The numbers of infiltrated PMNs were measured with MPO enzyme marker method and the myocardial infarction area was evaluated by TTC staining method. Results　After IR, myocardial levels of expression of ICAM-1 mRNA were increased significantly and reached a peak at 6 h; but the ICAM-1 protein peaked during 12-24 h. Number of PMNs and infarction size also increased and peaked after reperfusion 12-24 h. Close positive correlation (P<0.05) was found between the levels of expression of ICAM-1 protein, PMNs infarction area of the myocardium. In IR+NAC group, all of the indices were increased after IR, but the levels of increase in IR+NAC group were significantly lower (P<0.05 or 0.01) as compared with group IR (P<0.05-0.01). Conclusion　The findings indicate that PMNs adhesion and infiltration induced by ICAM-1 is implicated in the development of myocardial IRI. NAC could protect the myocardium from IRI by partially blocking the expression of ICAM-1.