山羊高原重度原发性肺冲击伤模型的建立

目的建立山羊在海拔4 600 m高原环境下的重度原发性肺冲击伤模型，分析高原重度原发性肺冲击伤的特点，为院前救治提供实验基础。 方法选取健康山羊28只，随机分为3 m组22只，3.5 m组6只，采用8 kg TNT当量新型爆炸物，在相同的环境条件下致伤，观察致伤后山羊肺冲击伤伤情，分别于伤前、伤后1 h、24 h监测山羊生命体征、肌钙蛋白Ⅰ，观察肺大体解剖，测肺组织重量、湿/干比重(W/D)等，对重伤山羊进行院前ABC救治。 结果伤后t≤15 min死亡12只，伤后15 min

Establishment of a model of severe primary lung blast injury in goats at high altitude

ObjectiveTo establish the model of severe primary lung blast injury in goats at an altitude of 4600 meters , and to provide experimental basis for the study of the characteristics and pre-hospital treatment of severe primary lung blast injury at high altitude. MethodsTwenty-eight healthy goats were randomly divided into two groups , 3 m group (n=22) and 3.5 m group (n=6). The lungs of goats were injured under the same environmental conditions with 8 kg TNT equivalent new explosives. The vital signs, troponin I, gross anatomy of lung, lung tissue weight and wet / dry specific gravity were observed before injury and 1 h, 24 h after injury. The seriously injured goats were treated with pre-hospital ABC. Results12 goats died within 15 minutes (including 15 minutes)after injury, survived more than 15 minutes after injury, but 4 goats died within 30 minutes (including 30 minutes), and the other 12 goats survived. According to the observation of the characteristics of severe primary lung blast injury, persistent respiratory distress, deep malaise, shivering, mouth, eye and nose bleeding, cough, hiccup and unstable standing appeared immediately after injury. The gross anatomy of the lungs of the two groups showed different degrees of pulmonary hemorrhage, pulmonary edema, pleural hemorrhage, pulmonary vesicles and pink foamed sputum in the airway, which were severe blast injury. The AIS score was 4.83 ±0.87. blood clots and a large amount of chyme could be seen in the main bronchus and branch trachea in part of the lungs. The lung injury of severe primary lung blast injury at high altitude is serious, and the on-site environment at high altitude is bad and complex, so it is difficult to treat the routine pre-hospital airway management and respiratory function maintenance program. ConclusionThe model of severe primary lung blast injury in goats at high altitude can be established by 8 kg TNT equivalent explosive at a distance of 3m and 3.5m. This model is stable and can be used to study the mechanism and pre-hospital treatment of primary lung blast injury at high altitude.