| 丝裂原活化蛋白激酶在醛固酮诱导心室成纤维细胞增殖中的作用 |
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| 引用本文： | 冯惠平,冯惠清,尹博英.丝裂原活化蛋白激酶在醛固酮诱导心室成纤维细胞增殖中的作用[J].临床心血管病杂志,2009,25(6). |
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| 作者姓名： | 冯惠平 冯惠清 尹博英 |
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| 作者单位： | 河北大学附属医院心内科,河北保定,071000 |
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| 摘 要： | 目的:探讨醛固酮诱导心肌成纤维细胞(CFs)增殖的信号转导机制.方法:用胰酶消化法分离、培养新生大鼠CFs,采用3H-胸腺嘧啶核苷(3H-TdR)掺入法、western-blotting技术从DNA合成、蛋白表达等方面观察MAPK活化与醛固酮促CFs增殖的关系.结果:①醛固酮剂量依赖性促CFs DNA合成,10-5mol/L PD98059 (782±152)个·min-1] 可逆转 10-7mol/L醛固酮 (1879±169)个·min-1]的作用;②10-7mol/L醛固酮对活化MAPK蛋白表达有显著增强作用,在4 h达峰作用(8.11±0.46),10-7mol/L 醛固酮的作用(8.11±0.46)被10-5mol/L PD98059 (1.21±0.10, P<0.01)阻断.结论:醛固酮能激活CFs的MAPK,MAPK表达及活化参与醛固酮诱导的CFs增殖.
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| 关 键 词： | 醛固酮 心肌成纤维细胞 丝裂原活化蛋白激酶 增殖 |
MAPK signal transduction mechanisms in the inductive effects on cardiac fibroblasts proliferation in neonatal rat induced by aldosterone |
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| FENG Huiping,FENG Huiqing,YIN Boying.MAPK signal transduction mechanisms in the inductive effects on cardiac fibroblasts proliferation in neonatal rat induced by aldosterone[J].Journal of Clinical Cardiology,2009,25(6). |
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| Authors: | FENG Huiping FENG Huiqing YIN Boying |
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| Institution: | Department of Cardiology;the Affiliated Hospital of Hebei University;Baoding;071000;China |
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| Abstract: | |
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| Keywords: | aldosterone cardiac fibroblasts mitogen-activated protein kinases proliferation |
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