Pro-inflammatory and (Epi-)genetic markers in saliva for disease risk in childhood obesity |
| |
| Institution: | 1. Ulm University Hospital, Division of Sports and Rehabilitation Medicine, 89075 Ulm, Germany;2. Institute for Human Genetics, Ulm University and Ulm University Medical Center, Ulm, Germany;1. Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, People''s Republic of China;2. Department of Biostatistics and Epidemiology, School of Public Health, Shenzhen University Health Science Center, Shenzhen, Guangdong, People''s Republic of China;1. Intermountain Medical Center Heart Institute, Salt Lake City, UT, USA;2. Division of Cardiovascular Medicine, Department of Medicine, Stanford University, Stanford, CA, USA;3. Cardiology Division and Nora Eccles Harrison CVRTI, University of Utah, Salt Lake City, UT, USA;4. Rocky Mountain University of Health Professions, Provo, UT, USA;5. Division of Cardiovascular Medicine, Department of Medicine, University of California San Diego, La Jolla, CA, USA;1. Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China;2. Department of Clinical Pharmacology, School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan, PR China;1. Institute for Community Medicine, University Medicine Greifswald, Greifswald, Germany;2. Department of Internal Medicine B – Cardiology, Intensive Care, Pulmonary Medicine and Infectious Diseases, University Medicine Greifswald, Greifswald, Germany;3. Department of Gastroenterology, University Medicine Greifswald, Greifswald, Germany;4. Institute of Diagnostic Radiology and Neuroradiology, University Medicine Greifswald, Greifswald, Germany;5. Institute for Laboratory Medicine and Clinical Chemistry, University Medicine Greifswald, Greifswald, Germany;6. German Centre for Cardiovascular Research (DZHK), Partner Site Greifswald, Germany;7. German Center for Diabetes Research (DZD), Partner Site Greifswald, Germany;8. Institute and Policlinic for Diagnostic and Interventional Radiology, University Hospital, Carl Gustav Carus University, TU Dresden, Dresden, Germany;9. Institute for Biometrics and Epidemiology, German Diabetes Center (DDZ), Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf, Düsseldorf, Germany;10. Department of Zoology, University of Malakand, 18800, Pakistan;11. Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Verona, Verona, Italy |
| |
| Abstract: | Background and aimChildhood obesity is an emerging problem often leading to earlier onset of non-communicable diseases in later life. Biomarkers to identify individual risk scores are insufficient in routine clinical practice, which is related to the need for easily sampled, non-invasive survey methods in children. We aimed to investigate and strengthen possible pro-inflammatory markers and epigenetic risk factors in saliva of obese children compared to lean controls.Methods and results19 overweight/obese (OC, 10.1 ± 1.9 years, BMI 27.7 ± 3.2 kg/m2) and 19 lean control children (CC, 9.7 ± 2.5 years, BMI 16.4 ± 1.8 kg/m2) participated in this explorative pilot study. Anthropometric measures, saliva and cheek swab samples were taken. Saliva profiles were examined for acute phase proteins (CRP and neopterin) and pro-inflammatory cytokines (IL-17a/IL-1β/IL-6). Cheek swabs were analyzed to investigate DNA methylation differences with subsequent hierarchical cluster and principal component analyses (PCA). Saliva analysis showed significant increased CRP concentrations in OC compared to CC (p < 0.001). There were no significant differences, but high intra-individual values in neopterin, IL-17a, IL-1β and IL-6. An unsupervised PCA of CpG loci with high variance (σ/σmax > 0.2) clearly separated OC and CC according to their methylation pattern. Furthermore, a supervised approach revealed 7125 significantly differentially methylated loci, whose corresponding genes were significantly enriched for genes playing roles in e.g., cellular signalling, cytoskeleton organization and cell motility.ConclusionsCRP and methylation status determinations in saliva are suitable as non-invasive methods for early detection of risks for non-communicable diseases in children/adolescents and might be a useful supplementary approach in the routine clinical practice/monitoring. |
| |
| Keywords: | Pediatric obesity Inflammation DNA methylation Epigenetics Cytokines |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
