| 重组果糖二磷酸醛缩酶SjLAP和亮氨酸氨基肽酶SjFBPA用于日本血吸虫病的诊断和疗效考核的评价(英文) |
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| 引用本文： | Faustina Halm-Lai,罗庆礼,钟政荣,宋晓蓉,陈兆武,王林,胡梅梅,储德勇,沈继龙.重组果糖二磷酸醛缩酶SjLAP和亮氨酸氨基肽酶SjFBPA用于日本血吸虫病的诊断和疗效考核的评价(英文)[J].中国寄生虫学与寄生虫病杂志,2011(5). |
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| 作者姓名： | Faustina Halm-Lai 罗庆礼 钟政荣 宋晓蓉 陈兆武 王林 胡梅梅 储德勇 沈继龙 |
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| 作者单位： | 安徽病原生物学省级实验室安徽省人兽共患病重点实验室安徽医科大学病原生物学教研室;Cape Coast大学微生物学教研室; |
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| 基金项目： | Supported by the National High Technology Research and Development Program of China(863 Program,No.2006AA02A251); the National Natural Science Foundation of China(No.81071376;No.30872209); the Doctoral Fund of Ministry of Education of China(No.200803660003)~~ |
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| 摘 要： | 目的评价重组亮氨酸氨基肽酶(rSjLAP)和重组果糖二磷酸醛缩酶(rSjFBPA)抗原用于诊断人血吸虫感染以及疗效考核的价值。方法异丙基-β-D-硫代半乳糖苷(IPTG)诱导pET-28a-rSjLAP/BL21和pET-28a-rSjFBPA/BL21表达目的蛋白,组氨酸标签亲和纯化柱纯化rSjLAP和rSjFBPA蛋白。88只BALB/c雌性小鼠随机分为4组,A、B和C组(各21只小鼠),D组(25只小鼠)。A、B和C组分别感染5、15和25条日本血吸虫尾蚴。D组为不感染对照组,在实验的第1天全部处死。A、B和C组在感染后第3、7、10、14、20、30和60天,分别处死小鼠3只,采眼球血制备血清,检测其抗体水平。采用单独或联合rSjLAP和rSjFBPA为抗原,ELISA法检测小鼠血清、急性血吸虫病(38份)和慢性血吸虫病患者(96份)血清中的抗体,以健康人(90份)血清为对照,同时检测华支睾吸虫病(33份)、卫氏并殖吸虫病(40份)和钩虫病患者(37份)血清,并检测急性血吸虫病患者吡喹酮治疗(60 mg/kg,2次/d×2 d)后1年的血清(36份)、慢性血吸虫病患者吡喹酮治疗(剂量,疗程同前)后1年...
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| 关 键 词： | 日本血吸虫病 免疫诊断 亮氨酸氨基肽酶 果糖二磷酸醛缩酶 |
Evaluation of Recombinant SjLAP and SjFBPA in Detecting Antibodies to Schistosoma japonicum |
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| Faustina Halm-Lai,LUO Qing-li,ZHONG Zheng-rong,SONG Xiao-rong,CHEN Zhao-wu,WANG Lin,HU Mei-mei,CHU De-yong,SHEN Ji-long Anhui Provincial Laboratory of Microbiology & Parasitology,Anhui Key Laboratory of Zoonoses.Evaluation of Recombinant SjLAP and SjFBPA in Detecting Antibodies to Schistosoma japonicum[J].Chinese Journal of Parasitology and Parasitic Diseases,2011(5). |
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| Authors: | Faustina Halm-Lai LUO Qing-li ZHONG Zheng-rong SONG Xiao-rong CHEN Zhao-wu WANG Lin HU Mei-mei CHU De-yong SHEN Ji-long Anhui Provincial Laboratory of Microbiology & Parasitology Anhui Key Laboratory of Zoonoses |
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| Institution: | Faustina Halm-Lai1,2,LUO Qing-li1,ZHONG Zheng-rong1,SONG Xiao-rong1,CHEN Zhao-wu1,WANG Lin1,HU Mei-mei1,CHU De-yong1,SHEN Ji-long1 1 Anhui Provincial Laboratory of Microbiology & Parasitology,Anhui Key Laboratory of Zoonoses,Department of Microbiology and Parasitology,Anhui,Medical University,Hefei 230022,China,2 Department of Microbiology,University of Cape Coast,Ghana |
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| Abstract: | Objective To investigate the early response of immunoglobulin G(IgG) antibody responses to Schistosoma japonicum infection in mice by using the recombinant proteins,S.japonicum leucine aminopeptidase(rSjLAP) and S.japonicum fructose-1,6-bisphosphate aldolase(rSjFBPA),and evaluate the potential of rSjLAP and rSjFBPA in diagnosis as well as in assessment of therapeutic efficacy in human schistosomiasis.Methods rSjLAP or rSjFBPA was induced from Escherichia coli BL21 strain transfected with the expression vect... |
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| Keywords: | Schistosomiasis japonica Immunodiagnosis Leucine aminopeptidase Fructose-1 6-bispho-sphate aldolase |
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