上海地区幽门螺杆菌菌株cag致病岛基因cagA、cagE、cagT检出率及临床意义

目的 检测上海地区幽门螺杆菌（Ｈｐ）分离株ｃａｇ致病岛（ＰＡＩ）中ｃａｇＡ、ｃａｇＥ、ｃａｇＴ基因，初步探讨ｃａｇＰＡＩ的完整性及其与胃十二指肠疾病的关系。方法 用多聚酶链反应（ＰＣＲ）技术扩增和检测了９９株从１７例慢性浅表性胃炎、２１例慢性性胃炎、１９例胃溃疡、２３例十二指肠溃疡和１９例胃癌中分离的Ｈｐ菌株的ｃａｇＡ、ｃａｇＥ、ｃａｇＴ基因。结果 ｃａｇＡ、ｃａｇＥ和ｃａｇＴ的总检出率分别为８４．８％（８４／９９）、９９．０％（９８／９９）和８４．８％（８４／９９），各基因检出率在各种胃十二指肠疾病患者分离的Ｈｐ菌株之间差异无显著性（均Ｐ〉０．０５）。９８株ｃａｇＥ阳性的菌株中，有１４株ｃａｇＡ阴性。结论 上海地区分离ｐ菌株绝大多数可能具有完整的ｃａｇＰＡＩ，其完整性与其感染后的临床结局不相关。ｃａｇＥ基因可

Prevalence of cagA, cagE and cagT genes in cag pathogenicity island of Helicobacter pylori strains isolated from Shanghai patients and their clinical implications

Objective To investigate the prevalence of the cagA, cagE and cagT, representative genes of cag pathogenicity island (PAI) in H. pylori strains isolated from patients with various gastroduodenal diseases in Shanghai and the relationship between the presence of these genes and gastroduodenal diseases. Methods 99 H. pylori strains isolated from 17 chronic superficial gastritis, 21 chronic atrophic gastritis, 19 gastric ulcer, 23 duodenal ulcer and 19 gastric cancer were studied for the prevalence of cagA, cagE and cagT genes by polymerase chain reaction (PCR). Results The total prevalences of cagA, cagE and cagT genes were 84.8%, 99.0% and 84.8%, respectively. There was no significant difference in the prevalence of all genes tested in H. pylori isolated from different gastroduodenal diseases (all P>0.05). Of 98 cagE positive isolates, 14(14.3%) were cagA negative. Conclusion Most of the H. pylori isolates in Shanghai region may have intact cag PAI. cagE, could be taken as the marker for the presence of cag PAI. There is no correlation between the integrality of cag PAI and the clinical outcome of H. pylori of infection.