A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis |
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| Authors: | Rita D Shelby Grace E Janzow Lauren Mashburn-Warren Jeffrey Galley Natalie Tengberg Jason Navarro |
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| Institution: | 1. Center for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital , Columbus, OH, USA;2. Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital , Columbus, OH, USA;3. Department of Pathology, Nationwide Children’s Hospital , Columbus, OH, USA |
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| Abstract: | ABSTRACT Clostridioides difficile infection (CDI) is a common cause of antimicrobial-associated diarrhea. Probiotics have shown variable results in decreasing its incidence and severity. We examined the efficacy of Lactobacillus reuteri administered using a novel probiotic biofilm delivery system in the treatment and prevention of CDI in a murine model. For prophylactic therapy, mice received an oral antibiotic cocktail followed by clindamycin injection, followed by probiotic administration (planktonic vs. biofilm state), followed by C. difficile oral gavage. For treatment therapy, mice received antibiotics and C. difficile first, followed by probiotic administration. Clinical sickness scores (CSS) and intestinal histologic injury scores (HIS) were assigned. |
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| Keywords: | C difficile probiotics lactobacillus reuteri biofilm therapeutic treatment prophylactic treatment |
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