TLC法对众生丸中蒲公英成分的快速鉴别

目的为建立众生丸中蒲公英成分的快速定性鉴别方法,采用薄层色谱法(TLC),基于样品与标准品对照,实现快速定性鉴别。方法通过参考有关文献,经过实验,对众生丸中蒲公英成分的提取方法、提取时间、提取溶剂、展开剂的选择、显色剂的选择对实验结果的影响进行研究。结果以乙酸乙酯为溶剂经超声提取、以三氯甲烷为展开剂,喷以10%硫酸乙醇溶液作为显色剂。薄层色谱鉴别斑点清晰,阴性对照无干扰。结论所建立方法操作简单,具有速度快,分离效果、专属性和重现性好的优点,应用于众生丸中蒲公英成分的快速定性鉴别,可加强对众生丸的质量控制。

Rapid Identification of Dandelion in Zhongsheng Pills by TLC

Objective To establish a rapid qualitative identification method of dandelion components in Zhongsheng pill, TLC was used to achieve rapid qualitative identification based on the comparison between samples and standard products. Methods Through reference to the literature and experiment, the effects of extraction method, extraction time, solvent extraction, development agent selection and color agent selection on the experimental results were studied. Results Ethyl acetate was extracted by ultrasound, trichloromethane was used as development agent, and 10 % ethanol sulfate solution was sprayed as color developer. TLC identified clear spots, negative control without interference. Conclusion The established method is simple in operation and has the advantages of fast speed, good separation effect, specificity and reproducibility. It can be applied to the rapid qualitative identification of dandelion components in Zhongsheng Pills and can enhance the quality control of Zhongsheng Pills.