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体外培养大鼠白内障模型晶状体的早期生化改变
作者姓名:Dong D  Lu A  Liu Y  Jia W  Hou W
作者单位:[1]首都医科大学附属北京同仁医院眼科 [2]北京医科大学生物化学与分子生物学系
摘    要:目的 进一步探讨白内障的发生机制。方法 采用器官培养方法,以不同浓度亚硒酸钠及半乳糖作用于晶状体,诱发大鼠晶状体形成白是,在培养初期测定晶状体内非蛋白质疏基(nonproteinsulfhydryl,NP-SH)、蛋白质疏基(protein sulfhydryl,P-SH)和不溶性蛋白质二硫键的含量,脂类过氧化水平,以及与谷胱甘肽代谢有关的谷胱甘肽过氧化物酶(GSH peroxidase,GSH-

关 键 词:白内障  组织培养  晶体  生物化学  大鼠

The early biochemical changes of cataractous lenses of rats cultured in vitro
Dong D,Lu A,Liu Y,Jia W,Hou W.The early biochemical changes of cataractous lenses of rats cultured in vitro[J].Chinese Journal of Ophthalmology,2000,36(5):344-347,I021.
Authors:Dong D  Lu A  Liu Y  Jia W  Hou W
Institution:Department of Ophthalmology, Tong Ren Hospital, Beijing 100073, China.
Abstract:Objective To study the mechanism of cataractogenesis Methods Tissue culture was used to study the cataractous lenses of rats induced by sodium selenite or galactose At the early stage, the content of nonprotein sulfhydryl (NP SH), protein sulfhydryl (P SH), non soluble disulfide bond, malonaldehyde (MDA) and the activities of glutathione peroxidase (GSH PX), glutathione reductase (GSSG R) and glutathione S transferase (GSH S) were measured in the cataractous lenses, and they were compared with that of the normal lenses Results The two kinds of material could all induce cataract in rats, sodium selenite being more potent In the early period of culture (lenses were transparent), NP SH and P SH were decreased, while disulfide and MDA were increased, the activity of GSH PX rose obviously, that of GSH S also had a tendency of rise, however, the activity of GSSG R had no obvious changes Conclusions Rat lens opacity may occur after the lens is cultured in vitro with the addition of sodium selenite or galactose, and biochemical changes may develop in the lens at the early period of culture (lenses are transparent)
Keywords:Cataract  Tissue culture  Lens  crystalline  Biochemistry
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