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鼠光感受器细胞层移植的方法及观察
作者姓名:Wang W  Ma M  Bai F  Zhang H
作者单位:北京医科大学第三医院眼科
摘    要:目的 研究视锥和视杆细胞与周围组织间在解剖结构及是生理等方面的关系。方法 取20只同龄Wistar和RCS鼠作为供体和受体,利用视见解发削技术取得视锥及视杆细胞层,并植入RCS鼠眼视网膜下。术后2周时摘除术眼,于光镜下观察交摄影。结果 术后2周,在视网膜完全复位的受体眼,移植的纯视锥和视杆细胞成活,可见移植细胞位于网膜下腔的视网膜色素上皮(retinal pigment epithelium,RP

关 键 词:细胞移植  光感受器  动物模型

A study on transplantation of rat photoreceptor cell layer
Wang W,Ma M,Bai F,Zhang H.A study on transplantation of rat photoreceptor cell layer[J].Chinese Journal of Ophthalmology,1999,35(6):443-445.
Authors:Wang W  Ma M  Bai F  Zhang H
Institution:Department of Ophthalmology, Third Hospital of Beijing Medical University, Beijing 100083.
Abstract:Objective To study the relationship between photoreceptors and other layers of the retina in the aspects of anatomical structure, physiology, pathology, etc . Methods RCS rats were used as host animals, and the pure cone and rod layer of the donor retina was prepared from Wistar rat and was transplanted under the host retina where the cones and rods were degenerated Results After 2 weeks of transplantation, the retinas of the host rats were reattached, and the transplanted cells were survival. The transplanted cells were in the subretinal space between the retinal pigment epithelium (RPE) and the inner unclear layer, and they had the staining characteristics similar to that of the normal control photoreceptos. Conclusion In the animal model of photoreceptor cell layer transplantation, the transplanted cells possess normal anatomical location and are connected with RPE and inner nuclear layer. The results provide a new route to investigate the re establishment of anatomical association with the optic nerve and the physiological activity of the nerve.
Keywords:Cell transplantation    Methods    Animal  laboratory
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