视网膜母细胞瘤p53、c-myc及增殖细胞核抗原表达的研究

目的 探讨Ｐ５３、ｃ－ｍｙｃ及增殖细胞核抗原在视网膜母细胞瘤中的表达及其与Ｒｂ分化和蔼和视神经浸润的关系。方法 应用抗人Ｐ５３、ｃ－ｍｙｃ及ＰＣＮＡ的单克隆抗体,采用锭霉卵白素－生物素免疫组化法,对３１例Ｒｂ常规石蜡标本行ｐ５３、ｃ－ｍｙｃ及ＰＣＮＡ表达的测定。结果 ｐ５３、ｃ－ｍｙｃ及ＰＣＮＡ在Ｒｂ中的表达阳性率分别为５１．６％、４５４．２％及６７．７％,且均与Ｒｂ的分化程度有关。ＰＣＮＡ的表达

A study of expression of p53, c-myc and PCNA in retinoblastoma

Objective To investigate the expressions of p53, cmyc (oncogene) and proliferating cell nuclear antigen (PCNA) in retinoblastoma (Rb) and the relationships of the expression to the degree of differentiation and optic nerve infiltration. Method The expressions of p53, cmyc and PCNA in Rb tissues of 31 cases were analyzed by using LSAB immunohistochemical method. Results The positive rates of p53, cmyc and PCNA in Rb were respectively 516%, 452% and 677% and all the expressions were significantly related to the differentiation degree of Rb (all P<005). The expression of PCNA was significantly related to the optic nerve infiltration of Rb (P<005). Both the expressions of p53 and cmyc in Rb were markedly correlated with the expression of PCNA (all P<005). Conclusions The occurrence of Rb is the result of multiple gene mutations. The determinations of p53, cmyc and PCNA are of significance for evaluating the histologic characteristics and biological behavior of Rb.