首页 | 本学科首页   官方微博 | 高级检索  
检索        

表皮生长因子受体家族在翼状胬肉上皮内的异常表达
作者姓名:Liu Z  Xie Y  Zhang M  Pflugfelder SC
作者单位:中山医科大学中山眼科中心,广州
摘    要:目的 了解表皮生长因子受体(epidermal growth factor receptor,EGFR)家族的EGFR、ErbB2及ErbB3蛋白在翼状胬肉上皮内的表达。方法 用免疫荧光组织化学及Western blot地15例初发期翼状胬肉患者切除的翼状胬肉组织进行EGFR、ErbB2及ErbB3蛋白的检测,并与正常人结膜组织进行对照。结果 免疫荧光组织化学染色显示,在正常结膜上皮中,EGFR蛋

关 键 词:翼状胬肉  表皮生长因子  发病机制  基因异常表达

The abnormal expression of epidermal growth factor receptor family in pterygium
Liu Z,Xie Y,Zhang M,Pflugfelder SC.The abnormal expression of epidermal growth factor receptor family in pterygium[J].Chinese Journal of Ophthalmology,2000,36(5):372-374,I025.
Authors:Liu Z  Xie Y  Zhang M  Pflugfelder S C
Institution:Email:zuguol@gzsums.edu.cn
Abstract:Objective To evaluate the expression of type 1 growth factor receptor family epidermal growth factor receptor (EGFR), ErbB2 and ErbB3] by the epithelium in pterygium so as to understand the pathogenesis of this disorder Methods The immunofluorescent staining and Western blotting were performed in 15 patients with primary pterygium, compared to normal conjunctival epithelium, to determine the expression of EGFR, ErbB2 and ErbB3 proteins Results The EGFR was present at the basal cells while the ErbB2 and ErbB3 were expressed by the superficial cells in normal conjunctival epithelium in immunofluorescent staining Of 15 pterygia, 11 pterygia expressed the stains of EGFR, ErbB2 and ErbB3 in the full thickness of epithelium and with stronger staining compared to the control group, 4 of them showed the same staining pattern as that in the normal conjunctiva Compared to normal conjunctiva, the stronger density of protein bands of EGFR, ErbB2 and ErbB3 was also demonstrated by western blotting in 11 pterygia with strong staining of these antibodies Conclusions The expression of EGFR, ErbB2 and ErbB3 is increased in pterygium, showing that the pterygium is a disorder with proliferation The abnormal expression of EGFR, ErbB2 and ErbB3 by epithelium and their communicating with cytokines at stroma in pterygium may be one of the key pathogenetic factors in this disorder
Keywords:Pterygium  Conjunctiva  Epidermal growth factor receptor  Pathogenesis
本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号