基因重组人表皮生长因子治疗兔角膜损伤的研究

目的 探讨基因重组人表皮生长因子（ｒｈＥＧＦ）对治疗角膜损伤的疗效。方法 新西兰白兔２４只,全麻下切除角膜前板层,直径８ｍｍ,约１／３角膜厚度。将其随机均分为４组,每组６只（１２只眼）,分别滴１、１０、１００μｇ／ｍｌ ｒｈＥＧＦ液,对照组滴生理盐水,每天４次,共７天。用计算机图像处理测量角膜损伤面积。结果 １、１０、１００μｇ／ｍｌ ｒｈＥＧＦ治疗组角膜上皮愈合速率分别为９．３１、９．９６、９．

An experimental research of recombinant human epidermal growth factor on corneal wound healing

OBJECTIVE: To investigate the effects of recombinant human epidermal growth factor (rhEGF) eye drops on corneal wound healing. METHODS: Twenty-four white rabbits were randomly divided into 4 groups, 6 rabbits 12 eyes each. Anterior keratectomy of 8 mm in diameter and 1/3 cornea in thickness was performed on each eye. Each of the following concentrations of rhEGF: 1, 10, 100 microg/ml eye drops or normal saline (control) was applied four times daily for a week respectively for one group. The wound area was determined by computer imaging analysis. RESULTS: The mean epithelial healing rate of rhEGF 1, 10, 100 microg/ml groups was 9.31, 9.96, 9.31 mm(2)/day respectively, significantly greater than 8.11 mm(2)/day of the control group. The action of rhEGF of 10 microg/ml was somewhat better than that of 1 or 100 microg/ml, and no significant difference was noticed among the three rhEGF groups. Moderate inflammation and corneal neovascularization were induced in the rhEGF 100 microg/ml treated group. CONCLUSION: rhEGF 1 - 10 microg/ml can accelerate corneal wound healing in the rabbit with no adverse side-effects. It may be used to treat serious corneal trauma and ulcer clinically.