鼠角膜碱烧伤的免疫学研究

目的 探讨角膜碱烧伤后眼局部的免疫反应机制。方法在大鼠角 制作碱烧伤模型。在烧伤后的不同阶段,制备角膜、虹膜组织平片,采用标准的卵白素－生物素过化物酶复合物免疫组方法。观察眼局部Ｔ淋巴细胞亚群,巨噬细胞、树突细胞、主要组织相容性复合物－Ⅱ类怕阳性细胞的动态变化。结果 碱烧伤后早期,角膜及虹膜即有Ｔ淋巴细胞浸润,以ＣＤ３淋巴细胞为主,ＭＨＣ－Ⅱ怕阳性细胞也轻度增多。在角膜溶解穿孔阶段,Ｔ淋巴细胞浸润

Immunologic experimental studies on the alkali burn of cornea in rats

OBJECTIVE: To investigate the immune reaction of severe corneal alkali burns. METHODS: The models of severe corneal alkali burns were induced in rats. Immunohistochemistry was carried out on wholemounts of the cornea and iris at different time points after corneal burns to detect T-lymphocytes and their subpopulation, macrophages, dendritic cells and MHC class II -positive cells. RESULTS: In the early stage of the burns, there were small increasing number of T-lymphocytes and MHC class II-positive cells in the cornea and iris. At the period of corneal melting and perforation, a massive influx of lymphocytes (CD(+)(3), CD(+)(4), CD(+)(8)), macrophages and dendritic cells was seen in the cornea adjacent to the limbus and iris. CD(+)(4) lymphocytes were much more than CD(+)(8) in number. T-cells and MHC class II-positive cells were observed not only at the limbus but also in the melting area and corneal center. Morphological changes of these cells were also noted. In the restoration stage, a number of positive cells were still found in the iris. CONCLUSION: Immunological mechanism is involved in the pathological changes of the damage induced by corneal alkali burns, especially in the pathogenesis of the corneal melting and perforation.