兔眼视网膜厚度的光学相干断层扫描测量与组织学检查的相关性研究

目的 探讨兔眼视网膜厚度的光学相干断层扫描(optical coherence tomography，OCT)检测与组织学检查结果的相关性。 方法 采用OCT对16只兔眼视网膜进行扫描，然后对相应部位的视网膜作组织切片检查。OCT成像截面采用人工辅助(需要检查者对反射界面进行人工定位)和电脑自动测量两种方法进行分析。 结果 两种OCT检查法测得的视网膜厚度与组织学检查结果有良 好的相关性。OCT人工辅助检查法(γ＝0.84，P＜0.001)较电脑自动测量方法(γ＝0.66，P＜0.01)的相关性略好。前者测量的误差范围( 95%可信限)较小，在－2．99～5.13μm之间，而后者则高达11.09μm。 结论 OCT能准确定量检测兔眼视网膜厚度；部分OCT成像截面可能因电脑机械测量而产生误差，通过人眼识别给予矫正后能提高检查的准确性。 （中华眼底病杂志，2000，16：71-138）

Correlation of retinal thickness measured by optical coherence tomography and histologic studies in rabbits

Purpose To evaluate the correlation of retinal thickness between optical coherence tomography (OCT) images and histologic slides ． Methods Retinal thickness was measured in 16 rabbit retinal histologic slides．The same eyes were previously viewed by OCT for the comparison of results between two methods．Retinal thickness of each OCT image section was measured using both the manually assisted (requiring observer localization of reflectivity peaks) and the automated modes of the computer software． Results Retinal thickness as measured by OCT demonstrated a high degree of correlation with retinal histologic study．The automate d method (γ=0．66，P＜0.01) was less reliable than the manually assisted one (γ=0．84，P＜0．001)．The former had an error in 95% confidence interval，ranged in-0.71～11.09 μm，the latter had a less error，ranged in-2.99～5.13μｍ． Conclusion Retinal thickness can be quantitatively measured by OCT examination．However，computer automatic identification of the reflective boundaries may result in errors in some cases．To measured the retinal thickness by manually assisted mode can increase the degree of accuracy．(Chin J Ocul Fundus Dis，2000，16：71-138)