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糖基化产物对牛视网膜微血管周细胞增生和胞浆[Ca2+] i 水平的影响
引用本文:席兴华,姜德咏,唐罗生.糖基化产物对牛视网膜微血管周细胞增生和胞浆[Ca2+] i 水平的影响[J].中华眼底病杂志,2000,16(3):169-172.
作者姓名:席兴华  姜德咏  唐罗生
作者单位:410011 长沙,湖南医科大学附属第二医院眼科
摘    要:目的 探讨糖基化产物对牛视网膜周细胞(pericyte,PC)增生和胞浆[Ca2+]i水平的影响。 方法 采用细胞计数结合噻唑蓝比色测定,氚标胸腺嘧啶核苷(3H-thymidine,3H-TdR)掺入和钙荧光探剂(Fura-2Acetoxymethyl ester,Fura-2AM),研究PC在牛血清白蛋白早期糖基化产物( early glycation products of bovine serum albumin,EG-BSA)和牛血清白蛋白糖基化终产物(advanced glycation end products of bovine serum albumin,AGE-BSA)培养下,PC增生数、DNA合成量及胞浆[Ca2+]i水平的改变。 结果 培养4 d后,细胞计数法:EG-BSA和AGE-BSA组PC数分别为17.87±2.36 ,14.77±3.72,较其对照组(20.54±0.82,20.31±0.93)减少13.00%和27.00%(P<0.01);MTT法:EG-BSA和AGE-BSA组分别为0.4619±0.0946,0.3884±0.1013,较其对照组(0.5236±0.0539,0.5227±0.0519)减少12.00%和25.70%(P<0.01);3H-TdR掺入量:EG-BSA和AGE-BSA组分别为39450.16±8870.68,33667.85±10581.70,较其对照组(56373.63±2317.97,56542.04±1961.23)减少30.00%和40.46%(P<0.01);胞浆[Ca2+]i水平:EG-BSA和AGE-BSA组分别为(129.55±30.41) nmol/L, (179.71±56.69) nmol/L,较其对照组[(79.70±6.94) nmol/L,(83.96±6.39) nmol/L ]增 高163.00%和214.00%(P<0.01)。 结论 EG-BSA和AGE-BSA能不同程度地抑制视网膜微血管PC增生和DNA合成,并使胞浆[Ca2+]i水平增高,尤以AGE-BSA为著。(中华眼底病杂志,2000,16:139-212)

收稿时间:1999-08-10
修稿时间:1999-08-10

Effects of glycation products on growth and cytosolic free calcium in bovine retinal capillary pericytes
XI Xing-hua,JIANG De-yong,TANG Luo-sheng..Effects of glycation products on growth and cytosolic free calcium in bovine retinal capillary pericytes[J].Chinese Journal of Ocular Fundus Diseases,2000,16(3):169-172.
Authors:XI Xing-hua  JIANG De-yong  TANG Luo-sheng
Institution:Department of Ophthalmology,the Second Affiliated Hospital,Hunan Medical University,Changsha 410011,China
Abstract:ObjectiveTo investigate the effects of glycation pro ducts on growth and cytosoic free calcium ([Ca2+]i) of bovine retinal capillary pericytes.MethodsThe changes of growth and [Ca2+]i of bovine retinal pericytes,which were cultured in early glycation products of bovine serum albumin (EG-BSA) and advanced glycation end products of bovine serum albumin (AGE-BSA),were studied by counting cell numbers,MTT colorimetric assay,[3H]thymidine incorporating,and fluorescent indicator fura-2 acetoxymeth1 ester (Fura-2AM). ResultsThe number of alive pericytes in groups of EG-BSA and AGE-BSA were 17.87±2.36 and 14.77±3.72 which comparing with their control groups (20.54±0.82 and 20.31±0.93)were de creased 13.00% and 27.00% (P<0.01) by counting cell numbers on a counting plate after four days.The results were 0.4619±0.0946 and 0.3884±0.1031 which comparing with their control groups (0.5236±0.0539 and 0.5227±0.0519)were decreased 12.00% and 25.70% (P<0.01) by MTT colorimetric assay.Amount of [3H]thymidine incorporating in groups of EG-BSA and AGE-BSA were 39450.16±887 0.68 and 33667.85±10581.70 which comparing with their control groups (56373.63±2317.97 and 56542.04±1961.23)were decreased 30.00% and 40.40% (P<0.01).The [Ca2+]i concentration of pericytes in groups of EG-BSA and AGE-BSA were (129.55±30.41) nmol/L and (179.71±56.69) nmo l/L which comparing with their control groups [(79.70±6.94) nmol/L and (83.±6.39) nmo l/L] were increased to 163.00% and 214.00%.ConclusionBoth EG-BSA and AGE-BSA can inhibit the proliferation and DNA syntheses of retinal capillary pericytes,and increased [Ca2+]i concentration in pericytes,especially the AGE-BSA.(Chin J Ocul Fundus Dis,2000,16:139-212)
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